Abstract：Objective：To explore the mechanism of Zhenqi Fuzheng granules in the treatment of liver cancer based on GEO database and network pharmacology. Methods： The differential genes of liver cancer tissues and adjacent normal tissues were obtained from GEO database，and the effective compounds and action targets of Zhenqi Fuzheng granules were obtained from Systematic Pharmacological Analysis Platform for Traditional Chinese Medicine(TCMSP). Effective compound target network was constructed， protein- protein interaction (PPI) network was constructed by STRING database， GO enrichment and KEGG pathway enrichment were analyzed by R software，and target signal pathway network diagram was constructed by Cytoscape 3.8.0 software. Results： Compared with adjacent normal tissues， the top three genes highly expressed in liver cancer tissue were GPC3， SPINK1 and AKR1B10， respectively. 87 active components of Astragalus membranaceus and 119 active components of Ligustrum lucidum were collected from TCMSP database. Network topology analysis showed that quercetin， kaempferol and luteolin were the top three compounds in center value and degree value， respectively. The top three potential target genes were CYP1A1，CYP3A4 and CYP1A2. A total of 273 biological process items were obtained by go enrichment. The biological process is mainly concentrated in the biological response to external stimuli， metal ions and cytokine metabolism. KEGG pathway is mainly enriched in xenobiotic metabolism of cytochrome P450， chemical carcinogenesis and drug metabolism-cytochrome P450. Conclusion ： Zhenqi Fuzheng granules can affect the biotransformation， metabolic clearance of chemotherapeutic drugs and improve the sensitivity to nanotherapy through multiple molecules， multiple targets and multiple pathways， so as to achieve the purpose of adjuvant treatment of liver cancer.