Abstract： Objective： To observe the clinical effect of Pudilan antiphlogistic oral liquid combined with recombinant interferon for children with hand- foot- mouth disease(HFMD) and its effects on creatine kinase MB(CK- MB) and cardiac troponin I(cTnI) in serum in children. Methods：The clinical data of 64 cases of HFMD children were retrospectively analyzed， and the children were divided into the control group and the treatment group according to the treatment methods in data， with 32 cases in each group. The control group was treated with recombinant human interferon α2b injection， and the treatment group was additionally treated with Pudilan antiphlogistic oral liquid based on the treatment of the control group. The levels of CK-MB，cTnI and inflammatory factors in serum before and after treatment in the two groups were compared；the clinical effects and improvement time of clinical symptoms after treatment in the two groups were compared；the adverse reactions in the two groups were compared. Results： The total effective rate was 93.75% in the treatment group， higher than that of 75.00% in the control group(P＜0.05). The antipyretic time，the subsided time of ulcer，oral mucosal congestion and the rash in the treatment group were shorter than those in the control group(P＜0.05). After treatment，the levels of CKMB， cTnI， tumor necrosis factor- α(TNF- α) and high- sensitivity C- reactive protein(hs- CRP) in serum in the two groups were decreased when compared with those before treatment(P＜0.05)，and the levels of interferon-γ(IFN-γ) in serum were increased(P＜0.05). After treatment，the levels of CK-MB，cTnI，TNF-α and hs-CRP in serum in the treatment group were lower than those in the control group(P＜0.05)，and the level of IFN-γ in serum was higher(P＜0.05). There was no significant difference being found in the comparison of the incidences of adverse reactions between the two groups(P＞0.05). Conclusion：The application of Pudilan antiphlogistic oral liquid combined with recombinant interferon for HFMD children can achieve a good clinical effect， which can timely relieve their clinical symptoms， reduce the levels of CK- MB， cTnI and inflammatory factors in serum，with fewer adverse reactions.