Abstract： Objective： To observe the clinical effect of tuina therapy of inducing yang into yin combined with breath guiding method on insomnia. Methods：A total of 60 patients with insomnia were randomly divided into the treatment group and the control group，with 30 cases in each group. The control group was treated with esazolam tablets，and the treatment group was treated with tuina therapy of inducing yang into yin combined with breath guiding method. Both groups were treated for 30 days. The clinical effect in the two groups were compared. Before and after treatment，the scores of Pittsburgh Sleep Quality Index(PSQI) and levels of 5- hydroxytryptamine(5- HT) and dopamine(DA) in serum in the two groups were compared. Results：The total effective rate was 93.33% in the treatment group，higher than that of 70.00% in the control group(P＜0.05). After treatment， the PSQI scores in the two groups were decreased when compared with those before treatment(P＜0.05)， and the PSQI score in the treatment group was lower than that in the control group(P＜0.05). After treatment，the levels of 5-HT in serum in the two groups were increased when compared with those before treatment(P＜0.05)， and the levels of DA in serum were decreased(P＜0.05)；the levels of 5-HT in serum in the treatment group was higher than that in the control group(P＜0.05)，and the levels of DA in serum was lower(P＜0.05). Conclusion：Tuina therapy of inducing yang into yin combined with breath guiding method has a good curative effect on patients with insomnia， which can effectively improve the sleep quality and regulate the neurotransmitter levels.