Abstract：Objective：To observe the effect of acupuncture combined with rehabilitation treating on signal transduction pathway of interleukin- 17(IL- 17) in peripheral blood mononuclear cells(PBMCs) in children with cerebral palsy. Methods：A total of 86 cases of children with cerebral palsy were divided into the control group and the acupuncture group according to the random number table method，with 43 cases in each group. The control group was treated with routine rehabilitation，and the acupuncture group was treated with routine rehabilitation combined with acupuncture. Both groups were treated for three months. The clinical effects in the two groups were observed. Before and after treatment，the changes of clinical spasm index (CSI)，Modified Ashworth Scale(Ashworth)，Berg Balance Scale(BBS)，Modified Falls Efficacy Scale(MFES)，6-min walking distance， 10- m walking time， IL- 17 content and mRNA expression levels of JAK2， STAT3 and IL- 17 in PBMCs were compared between the two groups. Results：After treatment，the total effective rate was 93.02% in the acupuncture group and 69.77% in the control group，the difference being significant(P＜0.05). After treatment，the scores of CSI and Ashworth in the two groups were decreased when compared with those before treatment， and the scores of BBS and MFES were increased；the scores of CSI and Ashworth in the acupuncture group were lower than those in the control group，and the scores of BBS and MFES were higher，differences being significant(P＜0.05). After treatment，the 1-min walking distances in the two groups were increased when compared with those before treatment， and the 10- min walking time was shortened；the 1-min walking distance in the acupuncture group was longer than that in the control group，and the 10-min walking time was shorter， differences being significant(P＜0.05). After treatment， the IL- 17 levels in serum in the two groups were decreased when compared with those before treatment，and the IL-17 level in serum in the acupuncture group was lower than that in the control group， differences being significant(P＜0.05). After treatment， the mRNA expression levels of JAK2，STAT3 and IL-17 in PBMCs in the two groups were decreased when compared with those before treatment， and the mRNA expression levels of JAK2，STAT3 and IL-17 in PBMCs in the acupuncture group were lower than those in the control group，differences being significant(P＜0.05). Conclusion：Based on routine rehabilitation treatment，the additional application of acupuncture can relieve clinical symptoms of cerebral palsy， and improve limb spasm， dysmyotonia and exercise function.