Abstract：Objective：To investigate the effects of lutein on proliferation，invasion and apoptosis of glioma cells and its possible mechanism. Methods：Low，medium and high doses of lutein were used to intervene glioma cells for 48 hours. Cell counting kit and Transwell method were used to detect cell viability and invasion，flow cytometry was used to detect cycle distribution and apoptosis，and real-time fluorescence quantitative PCR (RT-qPCR) was used to detect the presence of lutein α/β Hydrolase domain protein 11 antisense RNA1(ABHD11-AS1). The effects of interfering with the expression of ABHD11- AS1 on the proliferation，apoptosis and invasion of U251 cells were detected. Results：Low dose lutein had no significant effect on the survival，cycle distribution，invasion，apoptosis and ABHD11- AS1 expression of U251 cells. After treatment with medium and high concentrations of lutein，the survival rate，invasion number，S-phase cell proportion and ABHD11- AS1 expression of U251 cells decreased significantly，and the apoptosis rate and G0-G1 cell proportion increased significantly (P＜0.05). After interfering with the expression of ABHD11- AS1， the survival rate， invasion number and S- phase cell proportion of U251 cells decreased significantly，and the apoptosis rate and G0-G1 cell proportion increased significantly(P＜ 0.05). After overexpression of ABHD11-AS1，the survival rate，invasion number and S-phase cell proportion of U251 cells treated with lutein increased significantly，and the apoptosis rate and G0-G1 cell proportion decreased significantly(P＜0.05). Conclusion： A certain dose of lutein can reduce the proliferation and invasion of glioma cells and induce apoptosis. Its mechanism is related to the inhibition of ABHD11-AS1 expression.