Abstract： Objective： To analyze the molecular mechanism of astragaloside Ⅳagainst diabetic renal damage by using network pharmacology technology from the perspective of amino acid metabolism regulation. Methods：Based on previous metabonomics， amino acids related to the progression of diabetic nephropathy were screened. KEGG， PubChem and TCMSP databases were used to search and extract the effective target genes of amino acids and astragaloside Ⅳ，and KEGG pathway database was used for pathway analysis.The interaction network of astragaloside Ⅳ and amino acid gene target protein was drawn by Cytoscape，and the core gene target was extracted. The ClueGO inherent in Cytoscape3.7.1 software is used to enrich and analyze the pathway， biological process， cell localization and molecular function of amino acid gene targets related to disease progression， and draw the network diagram. Results： A total of 10 amino acids， which were closely related to the progression of diabetic nephropathy ， were analyzed. 82 of the target genes were extracted ， and 62 were involved in the KEGG pathway. 24 astragaloside gene targets were extracted. By constructing PPI network，fitting astragaloside Ⅳ and amino acid gene target network， 39 core targets were extracted. Enrichment analysis showed that astragaloside Ⅳ and amino acid core gene targets were involved in the regulation of biological processes such as cell stress， transcriptional growth factor regulation， protein deacetylation， RNA polymerase regulation， DNA binding regulation， cell cycle regulation， carbohydrate decomposition， glycolysis， cell proliferation and chromatin remodeling. The cell localization involved includes cyclin， RNA polymerase complex， euchromatin， transcription inhibitor， ATPase complex， PcG protein complex，histone methyltransferase，histone deacetylase，SWI/SNF superfamily complex，Sin3 complex and CHD complex. The molecular functions involved include regulating histone deacetylation，DNA transcription factor binding，RNA polymerase binding， transcription factor activation， chromatin DNA binding， ubiquitin protein binding， p53 binding， glucocorticoid receptor binding， etc.The KEGG pathway involved in the core gene target is mainly related to the regulation of cell cycle， cancer and autophagy pathway. Conclusion：AstragalosideⅣ may protect renal function at multiple molecular functional levels such as cell proliferation， cell cycle， oxidative stress and autophagy by regulating glucose metabolism， protein and DNA post transcriptional modification.