Abstract：Objective：To observe the clinical effect of dry needling therapy based on myofascial trigger points for neckshoulder myofascial pain syndrome. Methods：A total of 86 cases of patients with neck-shoulder myofascial pain syndrome were selected for prospective study，and were divided into the study group and the control group according to the random number table method，with 43 cases in each group. The control group was given routine electroacupuncture，and the study group was given dry needling therapy based on myofascial trigger points. Both groups were treated for three months. The clinical effects in the two groups were observed. Before and after treatment， the changes in Chinese medicine syndrome scores，cervical range of motion(CROM)，the pain degree in McGill Pain Questionnaire Scale(SF-MPQ)，the levels of tumor necrosis factor- α(TNF- α)， interleukin 1β(IL- 1β) and β- endorphin and substance- P in serum in the two groups were compared. Results： The total clinical effective rate was 95.35% in the study group and 81.40% in the control group， the difference being significant(P＜0.05). After treatment， the scores of Chinese medicine syndromes including pain， tenderness， swelling， dysfunction， stasis macules， tongue manifestation and pulse manifestation as well as the total scores in the two groups were decreased when compared with those before treatment(P＜0.05)，and the scores of Chinese medicine syndromes as well as the total score in the study group were lower than those in the control group，differences being significant(P＜0.05). After treatment， CROM levels of anteflexion， posterior extension， left lateral flexion and right lateral flexion in the two groups were increased when compared with those before treatment(P＜0.05)，and the above CROM levels in the study group were higher than those in the control group，differences being significant(P＜0.05). After treatment， SF-MPQ scores in the two groups were decreased when compared with those before treatment(P＜0.05)，and the SF-MPQ score in the study group was lower than that in the control group，differences being significant(P＜0.05). After treatment，the levels of TNF-α，IL-1β，and substance-P in serum in the two groups were decreased when compared with those before treatment， and the levels of β- endorphin in serum were increased， differences being significant(P＜0.05)； the levels of TNF-α，IL-1β，and substance-P in serum in the study group were lower than those in the control group，and the level of β- endorphin in serum was higher， differences being significant(P＜0.05). Conclusion： Dry needling therapy based on myofascial trigger points has a good curative effect in treating patients with neck-shoulder myofascial pain syndrome，which can effectively reduce the degree of pain and swelling and improve the cervical range of motion.