Abstract：Objective：To observe the neuroprotection effect of total Panax notoginseng saponin in rats with local cerebral ischemia. Methods： A total of 80 male SD rats were divided into the blank control group(Group A) and the non- blank rats group according to the random number table method， with 20 and 60 rats in each group respectively. The non- blank rats group were given model establishment. After successful establishment，the rats in the non-blank rats group were randomly divided into the ischemia model group(Group B)， the alteplase treatment group(Group C) and the treatment with alteplase combined with total Panax notoginseng saponin group(Group D)，with 20 rats in each group. There was no treatment given to Group A and B. Group C was given alteplase for injection，and Group D was additionally treated with total Panax notoginseng saponin based on the treatment of Group C，both groups were treated for five days. After the first treatment，the 24-hour neurological function scores in the four groups were compared. After treatment， the levels of malondialdehyde(MDA) and superoxide dismutase(SOD) in the brain tissue，and the levels of tumor necrosis factor-α(TNF-α) and interleukin-6(IL-6) in serum in the four groups were compared. Results：At 24 hours after the first treatment，the neurological function score in Group B was lower than that in Group A(P＜0.05)；the neurological function scores in Group C and D were higher than that in Group B(P＜0.05)，and the score in Group D was higher than that in Group C(P＜0.05). After treatment，the MDA level in brain tissue in Group B was higher than that in Group A(P＜0.05)，and SOD level was lower(P＜0.05)；the MDA levels in brain tissue in Group C and D were lower than that in Group B(P＜0.05)，and SOD levels in brain tissue were higher(P＜0.05)；the MDA level in brain tissue in Group D was lower than that in Group C(P＜0.05)， and SOD level was higher(P＜0.05). After treatment，the levels of TNF- α and IL-6 in serum in Group B were higher than those in Group A(P＜0.05)；the levels of TNF-α and IL-6 in serum in Group C and D were lower than those in Group B(P＜0.05)，and the two levels in serum in Group D were lower than those in Group C(P＜0.05). Conclusion： Total Panax notoginseng saponin can protect the neurological function in rats with local cerebral ischemia，and inhibit inflammatory responses and oxidative stress reactions.