Abstract： Objective： To explore the potential mechanism of Xuefu Zhuyu tang for secondary amenorrhea based on network pharmacology. Methods：The Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP) and ETCM database were used to search active ingredients of all medicines in Xuefu Zhuyu tang； DrugBank database， DisGeNET database and GeneCards database were used to search related targets of secondary amenorrhea； Cytoscape 3.8.0 software was used to construct the network of active ingredients of all Chinese herbs in Xuefu Zhuyu tang and corresponding targets (active ingredients- targets). The target protein- protein interaction(PPI) network between Xuefu Zhuyu tang and secondary amenorrhea was constructed and expanded by the database of STRING website， and the PPI network diagram was drawn by Cytoscape 3.8.0 software. Gene ontology(GO) classification enrichment analysis and Kyoto encyclopedia of genes and genomes(KEGG) pathway enrichment analysis were performed on the core targets through DAVID database. Results：A total of 163 targets of Xuefu Zhuyu tang were screened out，among which 34 targets were related to secondary amenorrhea，and a total of 64 related targets were obtained after amplification. Mainly by acting on interleukin-6， tumor suppressor P53 and other targets to regulate the function of steroids，tumor pathways and other biological processes and pathways，Xuefu Zhuyu tang plays a role in the treatment of secondary amenorrhea. Conclusion：Xuefu Zhuyu tang can act on multiple targets of secondary amenorrhea and participate in the regulation of multiple signaling pathways.