Abstract： Objective： To observe the effect of medicated serum of Xihuang pills on the proliferation， apoptosis and migration of human thyroid cancer cell TPC- 1. Methods： SPF New Zealand white rabbits were given 4， 8 and 16 g/kg of Xihuang pills for gastric irrigation to prepare medicated serum of Xihuang pills with low， medium and high- concentration， and the control serum was prepared，so as to treat human thyroid cancer cell line TPC-1 respectively. TPC-1 cells growing at logarithmic phase were selected and divided into the low， medium and high- concentration groups and the control group， with five replications in each group；the medicated serum of Xihuang pills with low，medium and high-concentration and the control serum were applied respectively for intervention. Methyl thiazolyl tetrazolium(MTT) was used to detect the proliferation of cells after intervention for 24， 48 and 72 hours respectively； flow cytometry and Transwell chamber experiment were used respectively to detect the apoptosis rate and the number of transmembrane cells after 48 hours of intervention；realtime fluorescence quantitative-polymerase chain reaction method was used to detect the mRNA of proliferating cell nuclear antigen(PCNA)，cell adhesion molecule 1(CADM1)，B-cell lymphoma- 2 gene(Bcl- 2) and Bcl- 2 associated X protein(Bax)； western blotting was used to detect the expression levels of PCNA，CADM1，Bcl-2，Bax，and Cleaved Caspase-3 protein. Results：The OD values of MTT experiment at different time points in the low，medium and high-concentration groups were lower than those in the control group(P＜0.05)； the OD values of MTT experiment in 48 and 72 hours in the mediumconcentration group were lower than those in the low-concentration group(P＜0.05)，and the OD values of MTT experiment at different time points in the high-concentration group were lower(P＜0.05)；the OD values of MTT experiment at different time points in the high- concentration group were lower than those in the medium- concentration group(P＜0.05). The apoptosis rates and the numbers of transmembrane cells in the low， medium and high- concentration groups were significantly increased when compared with those in the control group(P＜0.05)，with a concentration-dependent manner(P＜ 0.05). The relative expression levels of PCNA and Bcl-2 mRNA，and protein were lower in the groups of low，medium and high- concentration than in the control group(P＜0.05)， and the relative expression levels of Cleaved Caspase- 3 protein， CADM1，Bax mRNA and protein were higher(P＜0.05)；the relative expression levels of PCNA and Bcl-2 mRNA and protein in the medium and high- concentration groups were lower than those in the low- concentration group(P＜0.05)， and the relative expression levels of Cleaved Caspase- 3 protein， CADM1， Bax mRNA and protein were higher(P＜0.05)； the relative expression levels of PCNA and Bcl-2 mRNA and protein in the high-concentration group were lower than those in the medium- concentration group(P＜0.05)， and the relative expression levels of Cleaved Caspase- 3 protein， CADM1， Bax mRNA and protein were higher(P＜0.05). Conclusion：The medicated serum of Xihuang pills can inhibit the proliferation and migration of human thyroid cancer cell line TPC-1 and promote the apoptosis，among which the serum prepared with highconcentration Xihuang pills has the best effect. The mechanism may be related to down-regulation of PCNA and Bcl-2 mRNA and protein，up-regulation of CADM1 and Bax mRNA and protein，and Cleaved Caspase-3 protein expression.