Abstract： Objective： To investigate the effects of Ginsenoside Rg1 on the proliferation， invasion and secretion of inflammatory factors of fibroblast-like synoviocytes(FLSs)，so as to provide theoretical and experimental basis for the clinical treatment of rheumatoid arthritis(RA). Methods： FLSs of RA were isolated and cultured in vitro. FLSs were stimulated by tumor necrosis factor- α(TNF- α). Different concentrations of Rg1(0， 0.4， 4 and 40 mmol/L) were added in the above conditions. After 24 hours of culture，expression changes of interleukin-6(IL-6) and interleukin-1β(IL-1β) were detected by real-time fluorescence quantitative PCR(RT-PCR)，Western blot and Enzyme-linked immunosorbent assay(ELISA). CCK- 8 clone formation and Transwell evaluation of cell proliferation and invasion. The effect of Rg1 on the activation of mitogen activated protein kinase/extracellular regulated protein kinase(MAPK / ERK) pathway was evaluated by Western blot. Results： At the gene and protein levels， Rg1 could inhibit the expressions of IL- 1β and IL- 6 induced by TNF- α， and limit their secretion. In addition， Rg1 can significantly inhibit the invasion and proliferation of FLSs.In mechanism， Rg1 inhibited the phosphorylation of ERK and hindered the activation of MAPK/ERK pathway. Conclusion：Rg1 inhibits the activation of MAPK/ ERK pathway in FLSs，limits cell proliferation and invasion，and prevents inflammatory factors IL-6 and IL-1β. And thus play a role in the treatment of RA.