Abstract： Objective： To study the disease range and differentiation treatment rules related to Shaoyao Gancao tang syndrome in clinical medical cases， so as to provide theoretical basis and practice guidance for modern clinical application. Methods： The clinical medical cases concerning Shaoyao Gancao tang were searched from China National Knowledge Infrastructure，VIP Database and Wanfang Database from their constructions to Sep 2021. The main diseases，drug dosage and ratio，frequency of different doses，drug ratio and rules involved in Shaoyao Gancao tang were counted and analyzed. Results： A total of 103 disease kinds were involved， mainly concentrated on epigastric pain， gastrocnemius spasm， headache， constipation， hiccup， chronic gastritis， trigeminal neuralgia， abdominal pain， sciatica， dysmenorrhea， bronchial asthma and other diseases. The top three compatibility ratios of Radix Paeoniae Alba and Radix et Rhizoma Glycyrrhizae were 2∶1，3∶1 and 1∶1，accounting for 22.78%，18.51% and 8.01% respectively. The maximum dosage of Radix Paeoniae Alba ranged from 21 g to 30 g，and that of Radix et Rhizoma Glycyrrhizae ranged from 11 g to 20 g. In the treatment of digestive system diseases， the dosage of Radix Paeoniae Alba was mostly 21 g to 30 g， accounting for 34.59%，and that of Radix et Rhizoma Glycyrrhizae was mostly 1 g to 10 g，accounting for 46.49%；the compatibility ratio of 2∶1 was applied most，accounting for 21.08%. In the treatment of motor system diseases，the dosage of Radix Paeoniae Alba was mostly 21 g to 30 g， accounting for 39.68% ， and Radix et Rhizoma Glycyrrhizae was mostly 11 g to 20 g， accounting for 49.21%；the compatibility ratio of 2∶1 was applied most，accounting for 27.78%. In the treatment of nervous system diseases， the dosage of Radix Paeoniae Alba was mostly 21 g to 30 g， accounting for 37.76% ， and Radix et Rhizoma Glycyrrhizae was mostly 11 g to 20 g，accounting for 51.05%；the compatibility ratio of 2：1 appeared the most， accounting for 20.28%；In the treatment of respiratory diseases，the dosage of Radix Paeoniae Alba was mostly 11 g to 20 g， accounting for 33.33%，and Radix et Rhizoma Glycyrrhizae was 1 g to 10 g，accounting for 54.17%；the compatibility ratio of 2∶1 was applied most，accounting for 37.50%. In the treatment of urogenital diseases，the dosage of Radix Paeoniae Alba was mostly 21 g to 30 g，accounting for 36.36%，and Radix et Rhizoma Glycyrrhizae was mostly 1 g to 10 g，accounting for 65.45% ； the compatibility ratio of 3∶1 appeared the most， accounting for 23.64% . Conclusion： Shaoyao Gancao tang involves a wide range of diseases and is not confined to the treatment of one or several diseases. Due to the difference of pathogenesis，syndrome types and syndromes of different system diseases，the dose，ratio and role of prescriptions and drugs are naturally different. However， Shaoyao Gancao tang is considered as the key link to "treat diseases based on syndrome" and "apply prescriptions based on rules"， which also coincides with the thought of Zhongjing's six- channel syndrome differentiation，and provides new ideas for future researchers from the perspective of prescription and syndrome.