Abstract： Objective： To explore the effective components of the traditional Chinese medicine Polygonum cuspidatum and their possible mechanisms on Non- small Cell Lung Cancer(NSCLC) based on the method of network pharmacology. Methods： The active ingredients of Polygonum cuspidatum was screened and the drug target was precdicted in the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP). Relevant disease targets of NSCLC were screened in databases such as Online Mendelian Inheritance in Man(OMIM)， Therapeutic Target Database (TTD)，GeneCards and DrugBank.The Protein-Protein Interaction(PPI) network of core targets was constructed on the String database platform， the results were imported into cytoscape3.8.0 for analysis， AutoDockTools software was used to perform the molecular docking of active ingredients and core potential targets. Finally， Gene Ontology(GO) function enrichment and Kyoto encyclopedia of genes and genomes(KEGG) pathway analysis of potential core targets was performed on the Metascape gene function analysis website. Results： We finally acquired 18 active ingredients of Polygonum cuspidatum， 296 drug targets， and 179 potential core targets of Polygonum cuspidatum for NSCLC. The results of the network analysis shows that the key active ingredients of Polygonum cuspidatum for NSCLC include resveratrol，quercetin， apigenin， luteolin and emodin. The PPI network shows that the key targets include TP53， AKT1， CASP3， VEGFA， INS， and so on. The molecular docking results also show that there are good docking activities between the key active ingredients and key target proteins. GO function enrichment and KEGG pathway analysis showed that the pathways of Polygonum cuspidatum acting on NSCLC include classic cancer pathway，PI3K- Akt，MAPK，P53，TNF signaling pathway and so on. Conclusion：Polygonum cuspidatum contains multiple active ingredients，which can act on multiple targets through multiple pathways on NSCLC.