Abstract：Objective：To observe and study the effect of the therapy of Rupi Sanjie granules combined with postoperative adjuvant chemotherapy on the immune function and levels of tumor markers in patients who had undergone radical mastectomy for breast cancer. Methods： A total of 70 patients who had undergone radical mastectomy for breast cancer were randomly divided into the observation group and the control group， with 35 cases in each group. The control group was given chemotherapy of pirubicin and cyclophosphamide after surgery， and the observation group was additionally treated with Rupi Sanjie granules based on the treatment of the control group. Both groups were treated for four courses of treatment，21 days being one course. The levels of immunoglobulin A(IgA)，immunoglobulin G(IgG)，CD4+ cells and tumor markers，carcinoembryonic antigen(CEA)，carbohydrate antigen 125(CA125) and carbohydrate antigen 153(CA153)，the scores of quality of life in 36-Item Short Form Health Survey(SF-36) were compared between the two groups before and after treatment. Results：Before treatment，there was no significant difference in levels of IgA，IgG and CD4+ between the two groups(P＞0.05). After treatment，the levels of IgA，IgG and CD4 + in the observation group were higher than those in the control group，the difference being significant(P＜0.05). Before treatment， there was no significant difference in the levels of serum CEA， CA125 and CA153 between the two groups(P＞0.05). After treatment， the levels of CEA， CA125 and CA153 in both groups were decreased when compared with those before treatment(P＜0.05)，and the levels of CEA，CA125 and CA153 in the observation group were lower than those in the control group(P＜ 0.05). Before treatment，there was no significant difference being found in the comparison of the scores of SF- 36 between the two groups(P＞0.05). After treatment，the scores of SF- 36 in the two groups were increased when compared with those before treatment(P＜0.05)，and the scores in the observation group were higher than those in the control group. Conclusion： The therapy of Rupi Sanjie granules combined with chemotherapy of pirubicin and cyclophosphamide can effectively enhance the immune function of patients who had undergone radical mastectomy for breast cancer，reduce the levels of tumor markers in serum，and improve the quality of life of patients.