Abstract：Objective：To establish a thin-layer identification method for Radix Sophorae Flavescentis， Cortex Phellodendri Chinensis and Borneolum Syntheticum in Qingre Qushi lotion， and to investigate its quality stability. Methods：The Radix Sophorae Flavescentis，Cortex Phellodendri Chinensis and Borneolum Syntheticum in the prescription of Qingre Qushi lotion were identified by the method of thin- layer chromatography. The stability of Qingre Qushi lotion was investigated by influencing factors tests， accelerated tests and long- term tests. Results： The spots of Radix Sophorae Flavescentis， Cortex Phellodendri Chinensis and Borneolum Syntheticum in the thin- layer chromatograms were clear， with obvious characteristics and no interference from the negative control sample. Three batches of products showed good stability during long- term tests and accelerated tests， and the key inspection items of preparation stability met the standard. Conclusion：The preparation quality of Qingre Qushi lotion is stable and controllable， and the thin- layer chromatography method has good repeatability and high specificity， which can be used as the quality control method of Qingre Qudu lotion.