Abstract： Objective： To observe the effect of Qianyanqing prescription on Chinese medicine symptoms and levels of specific inflammatory cytokines in patients with chronic prostatitis. Methods： A total of 68 patients with chronic prostatitis were divided into the study group and the control group according to the random number table method， with 34 cases in each group. The control group was treated with routine therapy， and the study group was treated with Qianyanqing prescription. The curative effects， scores of Chinese medicine syndrome， National Institutes of Health- Chronic Prostatitis Symptom Index (NIH-CPSI) and Visual Analogue Scale(VAS) for pain and levels of serum specific inflammatory cytokines and prostatic fluid cytokines were compared between the two groups before and after treatment， and the occurrence of adverse reactions was recorded. Results： After treatment， the clinical effective rate was 97.06% in the study group，higher than that of 85.29% in the control group，there being no significance in the difference(P＞0.05). After treatment， the Chinese medicine syndrome scores of distending pain of perineum，lumbosacral spinal and testis，frequent urination，incomplete urination and white secretion of urethra，scores of NIH-CPSI and VAS，serum levels of interleukin-1β(IL-1β) and tumor necrosis factor-α (TNF-α)，and levels of interleukin-8(IL-8)，interleukin-10(IL-10) and transforming growth factor-1β(TGF- 1β) of prostatic fluid in the two groups were decreased when compared with those before treatment(P＜ 0.05)；the above scores and levels in the study group were lower than those in the control group(P＜0.05). Conclusion：The Qianyanqing prescription has a significant clinical effect on patients with chronic prostatitis， which can effectively alleviate the symptoms of patients，relieve the pain，and improve the level of specific inflammatory cytokines，with safety and reliability.