Abstract： Objective： To discuss the mechanism of Shenqi pills in the treatment of epithelialmesenchymal transformation(EMT) of renal tubules on the basis of the method of network pharmacology combined with differential gene analysis by GEO chip，and to further verify the targets of Shenqi Pill in the treatment of EMT of renal tubules by animal experiments. Methods： According to Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP) and BATMAN- TCM database， the active components and their corresponding targets of Shenqi pills were screened. The disease-related targets were obtained by GEO database and “drug- component- target” network was drawn by Cytoscape3 7.0 software. The interaction network of core target proteins was drawn by using STRING database and Cytoscape3 7.0 software，and R language was used to annotate the GO function and perform the pathway enrichment analysis of KEGG. The effects of Shenqi pills on threonine protein kinase 1(AKT1)， cell division cycle 20(CDC20)， epidermal growth factor receptor(EGFR)， nuclear receptor coactivator 1 (NCOA1) and cyclin B1(CCNB1) mRNA were verified by real- time fluorescence quantitative PCR. Results： A total of 229 active components of Shenqi pills were obtained， including 1 331 predicted targets， and 221 common targets with EMT of renal tubules on GEO chip. Shenqi pills mainly acted on AKT1，CDC20， EGFR，NCOA1，CCNB1 and other targets in the treatment of EMT of renal tubules. Go enrichment analysis involved many biological processes such as nutrient reactions， metal ion reactions and oxidative stress reactions. KEGG enrichment analysis obtained many signal pathways such as cAMP signal pathway， calcium signal pathway， thyroid hormone signal pathway， oxytocin signal pathway and HIF- 1 signal pathway. Real- time fluorescence quantitative PCR showed that Shenqi pills could increase the mRNA expression of AKT1 and reduce the mRNA expression of CDC20，EGFR and CCNB1 in the kidney tissues of adenine modle mice(P＜0.05). Conclusion： Based on the network pharmacology and experimental verification，it is preliminarily clarified that Shenqi pills can improve EMT of renal tubules by regulating the mRNA expression of AKT1， CDC20， EGFR and CCNB1， and its mechanism may be related to multiple signal pathways such as cAMP signal pathway and calcium signal pathway.