Abstract：Objective：To discuss the mechanism of Cinnamomi Ramulus-Paeoniae Radix Alba couplet medicinals in treating rheumatoid arthritis(RA) based on GEO gene chip， bioinformatics and molecular docking. Methods： The gene chip data set of RA was downloaded from the GEO database， and the differential expression targets were screened by R software；databases，such as TCMSP and PharmMapper， were used to obtain the chemical components and targets of Cinnamomi Ramulus- Paeoniae Radix Alba couplet medicinals，and Cytoscape 3.9.1 and R software were used to carry out the topology analysis of the protein-protein interaction(PPI) network of medicine-disease intersection targets ； packages ， including clusterProfiler package， were used to perform gene ontology(GO) and Kyoto Encyclopedia of Genes and Genomes(KEGG) enrichment analysis on the intersection targets； softwares， such as AutoDock Vina， were used to perform molecular docking for preliminary validation. Results： A total of 17 chemical components of medicine，3 781 RA targets and 410 medicine targets were obtained；a total of 98 medicinedisease intersection targets were screened out. The core targets of topology analysis of PPI included matrix metalloproteinase- 9(MMP-9) ， matrix metalloproteinase- 2(MMP-2) ， catalase(CAT) ， Serpin Family A Member 1(SERPINA1)， cathepsin B(CTSB) and so on； the main chemical compounds of Cinnamomi Ramulus- Paeoniae Radix Alba couplet medicinals in treating RA are taxiflin，β- sitosterol，(+ ) - catechin， paeoniflorin and so on. Potential targets involved in collagen decomposition and metabolism，extracellular matrix decomposition， inflammatory response regulation and other biological processes， involving pathways such as lysosomes， peroxisome proliferator- activated receptor(PPAR) signaling pathway and cytochrome P450 metabolism. Molecular docking showed that Cinnamomi Ramulus- Paeoniae Radix Alba couplet medicinals had high binding activity between the main chemical compounds and the core targets. Conclusion：The multi-target，multi-component and multi-pathway mechanism of Cinnamomi Ramulus- Paeoniae Radix Alba in treating RA provides a theoretical basis for the follow- up study of Cinnamomi Ramulus-Paeoniae Radix Alba couplet medicinals.