Abstract：Objective：To observe the clinical effect of the therapy of modified Danggui Shaoyao Powder combined with Daying-35 (Ethinylestradiol and Cyproterone Tablets) on polycystic ovary syndrome (PCOS) of kidney deficiency and blood stasis type and its effect on levels of serum sex hormone- binding globulin (SHBG) and adrenocorticotropic hormone (ACTH). Methods： A total of 92 PCOS patients of kidney deficiency and blood stasis type were selected as research objects and divided into the western medicine group and the combination group according to random number table method，with 46 cases in each group. The western medicine group was treated with Daying- 35， and the combination group was treated with modified Danggui Shaoyao Powder combined with Daying- 35. Both groups were treated for 3 courses of treatment. The clinical effects and the occurrence of adverse reactions were compared between the two groups. The changes of traditional Chinese medicine (TCM) syndrome scores，ovarian volume，endometrial thickness，endometrial protein expressions of phosphatidylinositol 3 kinase (PI3K)，protein kinase B (Akt)， phosphorylated Akt (p- Akt) and serum SHBG and ACTH levels before and after treatment were compared between the two groups. Results： The total effective rate was 93.48% in the combination group and 78.26% in the western medicine group， the difference being significant (P＜0.05). After treatment， the TCM syndrome scores of menstrual cycle， menstrual blood volumn， sore and weak lumbar and knee or lumbar spine trickling，dizziness and tinnitus，and abdominal pain during menstruation and the total scores in the two groups were all decreased when compared with those before treatment (P＜0.05)， and the above TCM syndrome scores and the total score in the combination group were lower than those in the western medicine group (P＜0.05). After treatment， the bilateral ovarian volume was decreased and endometrial thickness was increased in both groups when respectively compared with before treatment， differences being significant (P＜0.05). After treatment， the bilateral ovarian volume in the combination group was smaller than that in the western medicine group，and the endometrial thickness was bigger than that in the western medicine group，differences being significant (P＜0.05). After treatment，the relative protein expression levels of PI3K， Akt and P- Akt in the endometrium of the two groups were increased when compared with those before treatment (P＜0.05)，and those in the combination group were higher than those in the western medicine group (P＜0.05). After treatment，the serum SHBG levels in the two groups were increased when compared with those before treatment，and the ACTH levels were decreased when compared with those before treatment，differences being significant (P＜0.05). After treatment，the SHBG level in the combination group was higher than that in the western medicine group，and the ACTH level was lower than that in the western medicine group， differences being significant (P＜0.05). The incidence of adverse reactions was 4.35% in the combination group and 13.04% in the western medicine group，there being no significant difference between the two groups (P＞0.05). Conclusion：The therapy of modified Danggui Shaoyao Powder combined with Daying- 35 has a significant therapeutic effect on the treatment of PCOS of kidney deficiency and blood stasis type，which can alleviate clinical symptoms and correct sex hormone disorders by regulating PI3K/AKT signaling pathway and regulating reproductive axis.