Abstract：Objective：To observe the clinical effect of Jianpi Yishen Jiedu Sanjie Decoction combined with TP chemotherapy for advanced non- small cell lung cancer (NSCLC) and its effect on serum tumor markers and immune function. Methods： A total of 90 cases of patients with advanced NSCLC were selected and divided into the control group and the treatment group according to the random number table method， with 45 cases in each group. The control group was treated with TP chemotherapy， and the treatment group was additionally treated with Jianpi Yishen Jiedu Sanjie Decoction based on TP chemotherapy. Both groups were treated for two courses，with 21 days being a course of treatment. The clinical effects and the incidence of adverse reactions were compared between the two groups. The changes in serum tumor markers including carbohydrate antigen 199 (CA199)， cytokeratin 19 fragment antigen 21-1 (CYFRA21-1) and neuron-specific enolase (NSE)，and immune function (CD3+，CD4+，CD8+ and ratio of CD4+/CD8+) before and after treatment were compared between the two groups. Results：After treatment， the total clinical effective rate was 84.44% in the treatment group and 66.67% in the control group， the difference being significant (P＜0.05). After treatment， the levels of CA199， NSE and CYFRA21- 1 in the two groups were decreased when compared with those before treatment (P＜0.05)， and the levels of CA199，NSE and CYFRA21-1 in the treatment group were lower than those in the control group (P＜0.05). The levels of CD3 +，CD4 + and CD4 +/CD8 + in the treatment group were increased when compared with those before treatment， and the levels of CD8 + were decreased (P＜0.05)； the levels of CD3+，CD4+ and CD4+/CD8+ in the treatment group were higher than those in the control group (P＜0.05)， and the level of CD8+ was lower than that in the control group (P＜0.05). The incidence of adverse reactions was 24.44% in the treatment group and 57.78% in the control group，the difference being significant (P＜ 0.05). Conclusion： Jianpi Yishen Jiedu Sanjie Decoction combined with TP chemotherapy for advanced NSCLC can improve the clinical effect，inhibit the metastasis and proliferation of tumor cells，enhance the body immunity and reduce the incidence of adverse reactions，with high medication safety.