Abstract： Objective： To observe the clinical effect of acupuncture combined with western medicine thrombolytic therapy on the treatment of acute cerebral infarction (ACI) and its influence on the levels of inflammatory factors and nerve function. Methods：A total of 110 ACI patients were divided into the control group and the treatment group by random number table method，with 55 cases in each group. The control group was treated with thrombolytic therapy with Alteplase for injection， and the treatment group was additionally treated with acupuncture based on the treatment of the control group. The changes in the levels of inflammatory factors，including interleukin- 6 (IL- 6)，interleukin- 8 (IL- 8) and tumor necrosis factor- α (TNF- α)，National Institutes of Health Stroke Scale (NIHSS)，Barthel score，and hemorheology indexes， including hematocrit (HCT)，whole blood viscosity (WBV)，fibrinogen (Fib) and plasma viscosity (PV)，were compared between the two groups， and the clinical effects as well as adverse reactions were analyzed. Results： The total effective rate was 96.36% in the treatment group， higher than that of 83.64% in the control group， the difference being significant (P＜0.05). Before treatment， there was no significant difference in the levels of IL-6，IL-8 and TNF-α between the two groups (P＞0.05)；after treatment，the above levels in both groups were decreased when compared with those before treatment (P＜0.05)，and the above levels in the treatment group were lower than those in the control group (P＜0.05). Before treatment， there was no significant difference in the NIHSS score and Barthel score between the two groups (P＞0.05). After treatment，the NIHSS scores in the two groups were decreased when compared with those before treatment (P＜0.05)， and score of Barthel were increased when compared with those before treatment (P＜0.05)，and the NIHSS score in the treatment group was lower than that in the control group (P＜0.05)， and the scores of Barthel was higher than that in the control group (P＜0.05). Before treatment，there was no significant difference in the comparison of the levels of PV，Fib and HCT between the two groups (P＞0.05). After treatment， the above indexes in both groups were decreased when compared with those before treatment (P＜0.05)，and the indexes in the treatment group were lower than those in the control group (P＜0.05). The incidence of adverse reactions was 5.45% in the treatment group and 7.27% in the control group，there being no significant difference between the two groups (P＞0.05). Conclusion： The therapy of acupuncture combined with thrombolytic therapy with Alteplase has a significant therapeutic effect on the treatment of ACI patients，which can effectively control inflammation， improve blood circulation in brain tissue，promote the recovery of nerve function and improve the activities of dalily living of patients，with good safety.