Abstract： Objective： To observe the clinical effect of Lishi Shaoyao Prescription for ulcerative colitis with internal accumulation of damp- heat syndrome. Methods： A total of 64 cases of patients with ulcerative colitis with internal accumulation of damp-heat syndrome were divided into two groups according to the random number table method，with 32 cases in each group. The control group was treated with the oral administration of Mesalazin Enteric-Coated Tablets，and the treatment group was treated with the oral administration of Lishi Shaoyao Prescription. After 4-week administration，the scores of clinical symptoms including diarrhea， stool with mucus， stool with pus and blood， tenesmus， abdominal pain， and heat scorching the anus， the changes in the serum inflammatory factors including interleukin- 6 (IL- 6)， interleukin-17 (IL-17)，interleukin-4 (IL-4)，and tumor necrosis factor-α (TNF-α)，and the improvement of the Baron Scores for endoscopic grading and the Geboes Scores for mucosal histologic assessment were evaluated in the two groups. Results：The total clinical effective rate was 93.75% in the treatment group and 68.75% in the control group，the difference being significant (P＜0.05). After treatment，each score of clinical symptoms in the two groups was decreased when compared with that before treatment，and the scores in the treatment group were lower than those in the control group (P＜0.05). After treatment，the levels of IL- 6，IL- 17，and TNF- α，the Baron Scores，and the Geboes Scores in the two groups were decreased when compared with those before treatment， and the levels of IL- 4 were increased， differences being significant (P＜0.05). After treatment，the levels of IL-6，IL-17，and TNF-α，the Baron Scores，and the Geboes Scores in the treatment group were lower than those in the control group，and the IL- 4 level was higher than that in the control group， differences being significant (P＜0.05). Conclusion： Lishi Shaoyao Prescription for ulcerative colitis with internal accumulation of damp- heat syndrome can effectively reduce the levels of IL- 6，IL-17，and TNF-α，increase IL-4 level，relieve inflammation and intestinal mucosal ulcer， and improve the clinical symptoms including diarrhea，stool with mucus，stool with pus and blood，tenesmus，abdominal pain，and heat scorching the anus.