Abstract：Objective：To observe the clinical effect of Bushen Juanbi Decoction for knee osteoarthritis of liver and kidney deficiency type. Methods：A total of 38 cases of patients with knee osteoarthritis of liver and kidney deficiency type，who were treated from March 2019 to March 2021 in Gushi County Hospital of Traditional Chinese Medicine， were selected as the study subjects. Patients were treated with Bushen Juanbi Decoction. Before and after treatment， the clinical effect and the scores of Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) and 36-Item Short Form Health Survey (SF-36)，and the contents of Runt- related transcription factor 2 (RUNX- 2)，fibroblast growth factor 23 (FGF- 23)，and sex-determining region Y-related high-mobility group-box 9 (SOX-9) in serum were compared. Results： One patient was lost in follow- up， one patient fell off， and 36 patients were finally included in the statistics. After treatment ， 12 of the 36 patients showed significant difference，17 were effective，and 7 were ineffective，with the total effective rate being 80.56%. Compared with those before treatment，the WOMAC scores and the contents of RUNX- 2 and FGF- 23 in serum were significantly decreased (P＜ 0.05)，and the scores of SF-36 and the contents of SOX-9 in serum were significantly increased (P＜0.05). Conclusion：Bushen Juanbi Decoction has a good curative effect in treating knee osteoarthritis of liver and kidney deficiency type，which can effectively relieve the clinical symptoms and improve the quality of life of patients. Its mechanism is related to down- regulating the expression of RUNX- 2 and FGF- 23 and promoting the release of SOX-9.