Abstract： Objective： To observe the clinical effect of warming- needle moxibustion combined with tuina for knee osteoarthritis (KOA) and its effect on levels of osteoprotegerin (OPG) and osteocalcin (BGP) in serum. Methods： A total of 128 cases of KOA patients were divided into the control group and the observation group according to the random number table method，with 64 cases in each group. The control group was treated with Diclofenac Sodium， and the observation group was additionally treated with warming-needle moxibustion combined with tuina based on the treatment of the control group. Lequesne indexes，levels of OPG and BGP in serum before and after treatment as well as clinical effects in the two groups were compared. Results：Before treatment，there was no significant difference being found in the comparison of Lequesne indexes of pain and discomfort， walking ability and activities of daily living between the two groups (P＞0.05)；after treatment，the above scores in the two groups were decreased (P＜0.05)，and the scores in the observation group were lower than those in the control group (P＜0.05). Before treatment，there was no significant difference being found in the comparison of the levels of OPG and BGP in serum between the two groups (P＞0.05)； after treatment， the levels of OPG and BGP in serum between the two groups were increased (P＜0.05)， and the levels in the observation group were higher than those in the control group (P＜0.05). After treatment，the total effective rate was 93.75% in the observation group， significantly higher than that of 82.81% in the control group (P＜0.05). Conclusion： Warming-needle moxibustion combined with tuina for KOA can effectively increase the levels of OPG and BGP in serum，improve clinical symptoms and enhance clinical effects.