Abstract： Objective： To observe the clinical effect of the therapy of three- dimensional cervical manipulation combined with acupotomy on the treatment of cervical spondylosis radiculopathy and its influence on infrared thermal imaging of the diseased area. Methods：A total of 128 patients with radicular cervical spondylosis meeting the inclusion criteria were selected and divided into four groups according to random number table method，with 32 cases in each group. Group A was the control group，Group B was the three-dimensional cervical manipulation group，Group C was the acupotomy group，and Group D was the three-dimensional cervical manipulation combined with the acupotomy group. All the four groups were treated for three weeks. The changes of Neck Disability Index (NDI) and infrared thermal imaging were compared before and after treatment between the four groups. Results：After treatment and three months after treatment， the NDI scores in Groups B， C and D were compared with those before treatment， differences being significant (P＜0.05). After treatment and three months after treatment，the temperature differences in the neck of Groups B，C and D were compared with those before treatment，differences being significant (P＜0.05)； the temperature difference in the neck of Group D was decreased more significantly than that of Groups B and C，differences being significant (P＜0.05). There were no significant differences in the neck temperature difference of Group A after treatment and three months after treatment when compared with that before treatment (P＞0.05). Conclusion： The therapy of three- dimensional cervical manipulation combined with acupotomy can improve cervical function， whose clinical effect is better than that of simple three-dimensional cervical manipulation or acupotomy.