Abstract：Objective：To observe the clinical effect of warming-needle moxibustion combined with micro acupotomy for knee osteoarthritis (KOA). Methods： A total of 80 cases of KOA patients were divided into two groups according to the random envelope method， with 40 cases in each group. The control group was treated with routine warming-needle moxibustion ， and the observation group was additionally treated with micro acupotomy based on the treatment of the control group. Before and after treatment， the scores of clinical symptoms and Western Ontario and Mcmaster Universities Osteoarthritis Index (WOMAC)，range of motion (ROM) of knee joint，classification of fluid content，and serum indexes including interleukin-6 (IL-6)，matrix metalloproteinase-3 (MMP-3) and tissue inhibitor of metalloproteinases- 1 (TIMP- 1) were compared between the two groups. The adverse reactions were compared between the two groups. Results：Before treatment，there was no significant difference being found in the comparison of scores of clinical symptoms between the two groups (P＞0.05). After treatment， the scores of each clinical symptom were decreased (P＜0.05)， and the scores in the observation group were lower than those in the control group (P＜0.05). Before treatment，there was no significant difference being found in the comparison of scores of Lysholm and WOMAC，and ROM of knee joint between the two groups (P＞0.05). After treatment， the Lysholm scores in the two groups were increased (P＜0.05)，WOMAC scores were decreased (P＜0.05)，and ROM was enlarged (P＜0.05)；the Lysholm score in the observation group was higher than that in the control group (P＜0.05)，WOMAC score was lower than that in the control group (P＜0.05)，and ROM was larger than that in the control group (P＜ 0.05). Before treatment，there was no significant difference being found in the comparison of classification of fluid content between the two groups (P＞0.05). After treatment，the numbers of cases with Grade Ⅰ fluid content in the two groups were increased (P＜0.05)，and the numbers of cases with Grade Ⅲ and Ⅳ were decreased (P＜0.05)，and the difference between the two groups was statistically significant (P＜ 0.05). Before treatment，there was no significant difference being found in the comparison of levels of IL-6，MMP-3 and TIMP-1 in serum between the two groups (P＞0.05). After treatment，the levels of IL- 6 and MMP- 3 in serum in the two groups were decreased (P＜0.05)， and TIMP- 1 levels were increased (P＜0.05)；the levels of IL-6 and MMP-3 in serum in the observation group were lower than those in the control group，and TIMP-1 level was higher than that in the control group (P＜0.05). There was no adverse reactions in the two groups during treatment. Conclusion：Micro acupotomy combined with warming-needle moxibustion has a significant effect in treating KOA patients，which can improve clinical symptoms，knee joint function，and ROM of knee joint，reduce body inflammatory reaction，and delay chondrocyte degeneration，with certain safety.