Abstract：Objective：To observe the effect of Quyu Qingre Prescription on NLRP6/Caspase- 1/IL- 1β signaling pathway in rats with gouty arthritis (GA) induced by sodium urate. Methods：The swelling degree of ankle joint was detected. The contents of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) in serum were determined by enzyme- linked immunosorbent assay (ELISA). The contents of NLRP6 and Caspase-1 in synovial tissue were detected by Western Blot. Results：Compared with the normal group， the swelling degree of ankle joint in other groups was significantly increased at different time points after modeling (P＜0.05). Compared with the model group，the swelling degree of ankle joint in low-，mediumand high-dose groups of Quyu Qingre Prescription and colchicine group decreased at each time point after modeling (P＜0.05). Compared with colchicine group，the swelling degree of ankle joint in low-，mediumand high- dose groups of Quyu Qingre Prescription increased at each time point after modeling (P＜0.05). Serum levels of IL- 1β and TNF- α in model group were higher than those in normal group (P＜0.05). Compared with the model group，serum IL-1β and TNF-α levels were decreased in low-，medium- and high- dose groups of Quyu Qingre Prescription and colchicine group (P＜0.05). Compared with colchicine group，the concentration levels of IL- 1β and TNF- α in low-，medium- and high- dose groups of Quyu Qingre Prescription increased (P＜0.05). Compared with the normal group， NLRP6 protein expression in synovial tissue of model group and medium- and high-dose groups of Quyu Qingre Prescription was downregulated (P＜0.05)， and Caspase- 1 protein expression of model group was up- regulated (P＜0.05). Compared with the model group， NLRP6 protein expression was up- regulated and Caspase- 1 protein expression was down- regulated in synovial tissue of Quyu Qingre Prescription high- dose group (P＜ 0.05). Conclusion： Quyu Qingre Prescription can inhibit the inflammatory response of GA， reduce the content of IL- 1β and TNF- α in serum， up- regulate the expression of NLRP6 and down- regulate the expression of Caspase-1 protein in synovial tissue.