Abstract：Objective：To observe the effect of sequential external treatment of Ruyi Jinhuang Powder on wound and bacteria clearance rates after operation for perianal abscess. Methods：A total of 116 cases of patients after operation for perianal abscess were selected and divided into the observation group and the control group according to the random number table method， with 58 cases in each group. The control group was given fumigation with potassium permanganate and filled with vaseline gauze， and the observation group was given sequential external treatment of Ruyi Jinhuang Powder. The pain scores， scores of granulation tissue，levels of inflammatory factors，bacteria clearance rates，wound healing time were compared between the two groups. The clinical effects in the two groups were evaluated. Results： The total effective rate was 89.66% in the observation group， higher than that of 68.97% in the control group (P＜0.05). After treatment for 3， 8 and 14 days， the pain scores and granulation tissue growth scores in the observation group were lower than those in the control group (P＜0.05). After treatment，the levels of C- reactive protein (CRP) and interleukin 6 (IL- 6) in the two groups were decreased when compared with those before treatment (P＜0.05)，and the levels of CRP and IL-6 in the observation group were lower than those in the control group (P＜0.05). On the third day of treatment，the bacterial clearance rate was 31.03% in the observation group，higher than that of 8.62% in the control group (P＜0.05). On the eighth day of treatment， the bacterial clearance rate was 91.38% in the observation group， higher than that of 74.14% in the control group (P＜0.05). Conclusion：Sequential external treatment of Ruyi Jinhuang Powder can alleviate local pain， accelerate granulation tissue growth， reduce levels of inflammatory factors， accelerate bacterial clearance， shorten wound healing time， improve clinical treatment， and greatly alleviate patient pain after operation for perianal abscess.