Abstract：Objective：To study the expression of zinc finger transcription factor (GATA4) in the serum of patients with heart failure (HF) in coronary heart disease with qi deficiency and blood stasis syndrome and its correlation with clinical indexes，and to preliminarily formulate the diagnostic threshold for the syndrome. Methods： A total of 30 patients with HF in coronary heart disease with qi deficiency and blood stasis syndrome and 30 healthy people for control were collected. The enzyme- linked immunosorbent assay method (ELISA) was applied to detect the content of GATA4 in serum. Results：The content of GATA4 in serum of patients with HF with qi deficiency and blood stasis syndrome was 5 098.14±1 446.98 pg/mL， significantly higher than that of 2 819.28±1 022.74 pg/mL in the healthy control group，the difference being significant (P＜0.05). With the increase of heart function grading by the New York Heart Association (NYHA)， the serum GATA4 content in patients with HF with qi deficiency and blood stasis syndrome continues to increase， the difference being significant (P＜0.05). Serum GATA4 was positively correlated with NYHA heart function grading (r=0.914， P＜0.001)， positively correlated with BNP (r=0.697， P＜ 0.001)， and negatively correlated with left ventricular ejection fraction (LVEF) in HF patients with qi deficiency and blood stasis syndrome (r=- 0.592，P=0.001). The ROC was drawn to preliminarily draw the cut-off value of GATA4 in serum for diagnosing HF with qi stagnation and blood stasis syndrome，which was 3 777.49 pg/mL，and the area under the curve is 0.92. Conclusion：GATA4 can reflect the severity of HF and provide objective and quantitative reference and basis for HF with qi deficiency and blood stasis syndrome.