Abstract： Objective： To observe the effect of the therapy of Yishen Quyu Decoction combined with transdermal application with Chinese medicine on endometrial thickness and clinical pregnancy rate of retransfer of patients after in vitro fertilization- embryo transfer (IVF- ET) failure. Methods ： A total of 120 patients who failed in IVF- ET were selected and divided into the control group， the internal administration of Chinese medicine group， the transdermal application with Chinese medicine group and the combination group according to the random number table method，with 30 cases in each group. The control group was given oral administration of Vitamin E Soft Capsules； the transdermal application with Chinese medicine group was treated with transdermal application with Chinese medicine； the internal administration of Chinese medicine group was treated with oral administration of Yishen Quyu Decoction and the combination group was treated with internal administration of Chinese medicine and transdermal application with Chinese medicine. With one month as one course of treatment， the four groups were treated for three courses. The clinical pregnancy of the second- time IVF- ET after treatment in the four groups was counted；the traditional Chinese medicine (TCM) syndrome scores，scores of local signs and endometrial thickness were compared before and after treatment between the four groups；the safety of medication in the four groups was observed. Results：The clinical pregnancy rate in the combination group was higher than those in the other three groups (P＜0.05). After treatment，the TCM syndrome scores and scores of local signs in the four groups were lower than those before treatment (P＜0.05)， the above scores in the combination group were lower than those in the other three groups (P＜0.05)，and the above scores in the internal administration of Chinese medicine group and the transdermal application with Chinese medicine group were lower than those in the control group， respectively (P＜0.05). After treatment， the endometrial thickness in the four groups were higher than those before treatment (P＜ 0.05)，the endometrial thickness in the combination group was higher than those in the other three groups (P＜0.05)，and the endometrial thickness in the internal administration of Chinese medicine group and in the transdermal application with Chinese medicine group was higher than that in the control group，respectively (P＜0.05). Conclusion：The therapy of Yishen Quyu Decoction combined with transdermal application with Chinese medicine can improve the clinical pregnancy rate of re-transfer of patients after IVF-ET failure and improve the clinical symptoms and signs，whose mechanism may be related to the increase of endometrial thickness.