Abstract： Objective： To investigate the mechanism of chrysophanol regulating the Wnt/β- catenin signaling pathway to inhibit glioma cells. Methods： Human glioma cells U87 were cultured in vitro and treated with different concentrations of chrysophanol (0，50，100，200 μmol/L). The proliferation capacity of U87 cells was detected by CCK- 8 method. Cell migration ability was detected by scratch method. Cell invasion ability was detected by Transwell method. Flow cytometry was used to detect apoptosis. Western Blot was used to detect the expressions of apoptosis- related proteins Bax， Bcl- 2 and β- catenin， Ecadherin， vimentin and MMP- 9 proteins related to Epithelial- mesenchymal transition (EMT) in Wnt pathway. Results：Rhein could inhibit proliferation，migration and invasion of U87 cells，induce apoptosis (P＜0.05)，down-regulate the expression levels of Bcl-2，β-catenin，vimentin and MMP-9，and upregulate the expression levels of Bax and E- cadherin (P＜0.05)， in a dose- dependent relationship. Conclusion：Chrysophanol can inhibit the proliferation，migration and invasion of glioma cells U87，and its mechanism may be related to the inhibition of Wnt/β-catenin signaling pathway.