Abstract： Objective： To observe the effect of Stroke Prescription Ⅱ combined with acupuncture on stroke with qi deficiency and blood stasis syndrome and its effect on learning and memory function， cerebral blood flow and short- chain fatty acids. Methods： A total of 140 cases of stroke patients with qi deficiency and blood stasis syndrome were selected and divided into the combination group and the routine group according to the random number table method，with 70 cases in each group. The routine group was treated with the Stroke Prescription Ⅱ，and the combination group was treated with acupuncture based on the treatment of the routine group. The clinical effects and disability status were compared in the two groups. The changes in traditional Chinese medicine (TCM) syndrome scores， learning and memory function，Activities of Daily Living (ADL) scores，National Institutes of Health Stroke Scale (NIHSS) scores， cerebral blood flow，and short-chain fatty acids were compared before and after treatment. Results：The total effective rate of clinical effect was 94.29% in the combination group， and 82.86% in the routine group，the difference being significant (P＜0.05). After treatment，the scores of TCM syndromes including hemiplegia， tongue and mouth deviation， awkward speech， and numbness in the two groups were decreased when compared with those before treatment (P＜0.05)，and the above TCM syndrome scores in the combination group were lower than those in the routine group (P＜0.05). After treatment，the minimum blood flow velocity，maximum blood flow velocity，average blood flow velocity，and average blood flow in the two groups were increased when compared with those before treatment (P＜0.05)，and the cerebral vascular resistance was decreased when compared with that before treatment (P＜0.05)； the minimum blood flow velocity，maximum blood flow velocity，average blood flow velocity，and average blood flow in the combination group were higher than those in the routine group (P＜0.05)， and the cerebral vascular resistance was lower than that in the routine group (P＜0.05). After treatment， there was no significant difference in the comparison of isobutyric acid，valeric acid，isovaleric acid，and caproic acid between the two groups (P＞0.05). After treatment， the levels of acetic acid， propionic acid， butyric acid， and total short chain fatty acids in the two groups were increased when compared with those before treatment (P＜ 0.05)； the levels of acetic acid， propionic acid， butyric acid， and total short chain fatty acids in the combination group were higher than those in the routine group (P＜0.05). After treatment， the clinical memory scale scores and ADL scores in the two groups were increased when compared with those before treatment (P＜0.05)，and the NIHSS scores were decreased when compared with those before treatment (P＜0.05). The clinical memory scale scores and ADL scores in the combination group were higher than those in the routine group (P＜0.05)，and the NIHSS score was lower than that in the routine group (P＜ 0.05). The degree of disability in the combination group was lower than that in the routine group， the difference being significant (P＜0.05). Conclusion：Stroke Prescription Ⅱ combined with acupuncture has a significant curative effect for stroke with qi deficiency and blood stasis syndrome，which can promote the recovery of cognitive function， improve the patient's learning and memory function， alleviate clinical symptoms，improve cerebral blood flow indexes，and reduce disability.