Abstract：Objective：To observe the curative effect of midnight-midday ebb flow chrono-acupuncture combined with conventional therapy for vascular cognitive impairment of none dementia (VCIND). Methods： A total of 110 cases of VCIND patients were selected and divided into the control group and the observation group according to the random number table method，with 55 cases in each group. The control group was treated with conventional western medicine treatment，and the observation group was additionally treated with midnight-midday ebb flow chrono-acupuncture based on the treatment of the control group. Clinical effects and the incidence of adverse reactions were compared between the two groups. The changes in traditional Chinese medicine syndrome (TCM) scores，cerebral hemodynamic indicators [blood flow velocity (Vm)， fastest diastolic blood flow velocity (Vd)， and fastest systolic blood flow velocity (Vs)]， cognitive function [Montreal Cognitive Assessment (MoCA) and Mini-Mental State Examination (MMSE)]， levels of serum-related factor [serum brain-derived neurotrophic factor (BDNF)， homocysteine (Hcy)， and glial fibrillary acidic protein (GFAP)] were compared between the two groups before and after treatment. Results： The total clinical effective rate was 92.45% in the observation group and 75.00% in the control group，the difference being significant (P＜0.05). After treatment， TCM syndrome scores in the two groups were decreased when compared with those before treatment (P＜0.05)， and the TCM syndrome scores in the observation group were lower than those in the control group (P＜0.05). After treatment，the levels of Vs， Vd，and Vm in the two groups were increased when compared with those before treatment (P＜0.05)，and the levels of Vs， Vd， and Vm in the observation group were higher than those in the control group (P＜ 0.05). After treatment，the scores of MoCA and MMSE in the two groups were increased when compared with those before treatment (P＜0.05)，and the scores of MoCA and MMSE in the observation group were higher than those in the control group (P＜0.05). After treatment， the levels of BDNF in the two groups were increased when compared with those before treatment， and the levels of Hcy and GFAP were decreased when compared with those before treatment (P＜0.05)； the levels of Hcy and GFAP in the observation group were lower than those in the control group (P＜0.05)， and the level of BDNF in the observation group was higher than that in the control group (P＜0.05). The incidence of adverse reactions was 5.66% in the observation group and 9.62% in the control group， there being no significance in the difference (P＞0.05). Conclusion：Midnight-midday ebb flow chrono-acupuncture has significant effects in the treatment of VCIND， which can reduce nerve cell damage， improve brain blood circulation， and enhance cognitive function，with high safety.