Abstract： Objective： To observe the clinical effect of electroacupuncture at point selection of antagonistic muscle for post-stroke motor dysfunction. Methods：A total of 97 cases of patients with poststroke motor dysfunction were selected as the research subjects and divided into the observation group and the control group according to the random number table method，with 48 cases in each group. Both groups received routine basic treatment； the control group received routine rehabilitation treatment， and the observation group received electroacupuncture at point selection of antagonistic muscle based on the treatment of the control group. Both groups were treated for 8 weeks. The clinical effects and incidence of adverse drug reactions were compared between the two groups. Before and after treatment，the changes in traditional Chinese medicine (TCM) syndrome scores， joint motion， limb movement function [Fugl- Meyer Assessment Scale，Berg Balance Scale (BBS)，and Barthel Index (BI)]，electromyographic signals， and serum neurological factors [glycine (Gly)，γ-aminobutyric acid (GABA)，neuron specific enolase (NSE)， and neuropeptide Y (NPY)] were compared between the two groups. Results： After treatment， the total clinical effective rate was 93.88% in the observation group，higher than that of 77.08% in the control group (P＜0.05). After treatment， the TCM syndrome scores in the two groups were decreased (P＜0.05)， and the score in the observation group was lower than that in the control group (P＜0.05). After treatment，the range of motion of the shoulder，elbow，hip，knee，and ankle was increased in the two groups (P＜0.05)， and the one in the observation group was greater than that in the control group (P＜0.05). After treatment， the Fugl-Meyer scores，BBS scores，and BI scores in the two groups were increased (P＜0.05)，and the scores in the observation group were higher than those in the control group (P＜0.05). After treatment，the electrical signals of the biceps brachii and tibialis anterior muscles in the two groups were increased (P＜ 0.05)，and the ones in the observation group were greater than those in the control group (P＜0.05). After treatment，the levels of GABA and Gly in serum in the two groups were increased (P＜0.05)，and the levels in the observation group were higher than those in the control group (P＜0.05). The levels of NSE and NPY in serum in the two groups were decreased (P＜0.05)， and the levels in the observation group were lower than those in the control group (P＜0.05). During the treatment period， there were no significant adverse reactions in the two groups. Conclusion：The electroacupuncture at point selection of antagonistic muscle can effectively alleviate the clinical symptoms of patients with post-stroke motor dysfunction， improve joint motion and limb function，and promote neurological recovery.