Abstract：Objective：To observe the clinical effect of Guipi Decoction and Sini Powder combined with acupuncture on multiple tics in children of spleen deficiency and liver effulgence type. Methods：A total of 70 cases of children with multiple tics of spleen deficiency and liver effulgence type were selected as the research objects， and were divided into the study group and the control group according to the random number table method， with 35 cases in each group. Both groups were given routine symptomatic treatment； the control group was treated with acupuncture ， and the study group was additionally treated with Guipi Decoction and Sini Powder based on the treatment of the control group. Both groups were treated for three months. The clinical effects in the two groups were evaluated； traditional Chinese Medicine (TCM) syndrome scores， Yale Global Tic Severity Scale (YGTSS) scores and serum neurotransmitter levels before and after treatment were compared between the two groups；the incidence of adverse reactions were counted. Results：There was no significant difference in the comparison of the total effective rates between the two groups (P＞0.05). After treatment，TCM syndrome scores，motor tic， vocal tic and total scores of YGTSS in the two groups were decreased when compared with those before treatment (P＜0.05)， and the scores in the study group were lower than those in the control group (P＜ 0.05). After treatment， γ -aminobutyric acid (GABA) levels in the two groups were increased， and the levels of dopamine (DA) and norepinephrine (NE) were declined when compared with those before treatment (P＜0.05). After treatment， GABA level in the study group was higher than that in the control group， and the levels of DA and NE were lower than those in the control group (P＜0.05). There was no significant difference in the incidence of adverse reactions between the two groups (P＞0.05). Conclusion： Guipi Decoction and Sini Powder combined with acupuncture has a definite curative effect in the treatment of multiple tics in children of spleen deficiency and liver effulgence type， which can improve the tic symptoms and regulate neurotransmitter levels，with high safety.