Abstract：Objective：To observe the clinical effect of mind-regulating acupuncture method combined with Estazolam Tablets on insomnia complicated with depression and its effect on 5-hydroxytryptamine (5-HT) and dopamine (DA). Methods：A total of 98 patients with insomnia and anxiety were divided into the treatment group and the control group according to the random number table method， with 49 cases in each group. The control group was treated with Estazolam Tablets， and the treatment group was treated with mind-regulating acupuncture method and Estazolam Tablets. Both groups were treated continuously for four weeks. The scores of Pittsburgh Sleep Quality Index (PSQI)， Insomnia Severity Index (ISI)， Hamilton Anxiety Scale (HAMA)，as well as levels of sleep structural parameters [sleep latency，rapid eye movement (REM) latency， total sleep time， awakening time， and sleep efficiency]， and serum neurotransmitters (5-HT， DA) were compared between the two groups before and after treatment. The clinical efficacy and adverse reactions were evaluated in the two groups. Results： After treatment， the total effective rate was 95.92% in the treatment group，higher than that of 83.67% in the control group (P＜ 0.05). After treatment， the scores of PSQI， ISI， and HAMA， sleep latency， REM latency， and awakening time and serum DA levels in the two groups were decreased when compared with those before treatment (P＜0.05)， and the scores of PSQI， ISI， and HAMA， sleep latency， REM latency， and awakening time and serum DA levels in the treatment group were lower than those in the control group (P＜ 0.05). After treatment，the total sleep time and sleep efficiency and serum 5-HT levels in the two groups were increased when compared with those before treatment (P＜0.05)，and the total sleep time and sleep efficiency and serum 5-HT levels in the treatment group were higher than those in the control group (P＜ 0.05). There was no significant difference in the incidence of adverse reactions between the two groups (P＞ 0.05). Conclusion： Mind-regulating acupuncture method combined with Estazolam Tablets has a definite curative effect on insomnia with anxiety，which can regulate the patient's neurotransmitter levels，improve sleep quality，and alleviate anxiety，with high safety.