Abstract：Objective：To observe the clinical effect of the therapy of Peiyuan Tiaoshen acupuncture and moxibustion method combined with midnight-midday point application on premature ovarian failure and its effect on ovarian volume and blood flow parameters of ovary. Methods： A total of 92 patients with premature ovarian failure were divided into the control group and the treatment group according to the random number table method， with 46 cases in each group. The control group was treated with routine western medicine， and the treatment group was additionally treated with Peiyuan Tiaoshen acupuncture and moxibustion method combined with midnight-midday point application based on the treatment of the control group. The traditional Chinese medicine (TCM) syndrome scores， Anxiety Self-Rating Scale (SAS) score， ovarian volume， number of ovarian follicles， blood flow parameters of ovary and levels of serum sex hormones between the two groups were compared before and after treatment，and the clinical effects and incidence of adverse reactions in the two groups were evaluated. Results：After treatment，the total effective rate was 91.30% in the treatment group， higher than that of 73.91% in the control group (P＜ 0.05). After treatment， the TCM syndrome scores， SAS score， resistance index and levels of serum luteinizing hormone (LH) and follicle-stimulating hormone (FSH) in the two groups were decreased when compared with those before treatment (P＜0.05)，and the TCM syndrome scores，SAS score，resistance index and levels of serum LH and FSH in the treatment group were lower than those in the control group (P＜0.05). After treatment，the levels of estradiol (E2)，and peak systolic velocity and end diastolic velocity of ovary in the two groups were increased when compared with those before treatment (P＜0.05)；the left and right ovarian volume was increased (P＜0.05)，and the number of ovarian follicles was increased (P＜ 0.05)； the above indexes in the treatment group were higher than those in the control group (P＜0.05). There was no adverse reaction in the two groups. Conclusion： The therapy of Peiyuan Tiaoshen acupuncture and moxibustion method combined with midnight-midday point application has a definite curative effect on premature ovarian failure， which can regulate the levels of sex hormones of patients， and improve the blood flow parameters of ovary with great safety.