Abstract： Objective： To discuss the molecular mechanism of Wubei Powder in treating reflux esophagitis (RE) and gastric ulcer (GU) with different diseases， same treatment. Methods： Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP)， HERB database， SymMap database， and BATMAN-TCM database were used to screen the main active ingredients and targets of Wubei Powder； GeneCards and OMIM database were used to obtain two disease-related targets and take the intersection to obtain common targets between the medicine and diseases；the target intersections were imported into the STRING database to construct a protein interaction network， and Cytoscape software was used for visualization processing and obtaining key targets. Gene Ontology (GO) functional analysis on common targets was performed with the DAVID database，and pathway enrichment analysis on common targets was performed with the Kyoto Encyclopedia of Genes and Genomes (KEGG)；a diagram of effective ingredients， target intersection， and pathways was constructed； molecular docking verification was performed on key targets and main active ingredients. Results： A total of 20 active ingredients were obtained from Wubei Powder for the treatment of RE and GU， among which the main active ingredients were quercetin， geranium extract， and β -sitosterol； there were 730 intersection disease targets between RE and GU，and 92 intersection targets between Wubei Powder and the diseases. The key targets of Wubei Powder in the treatment of RE and GU included S non-receptor tyrosine kinase (SRC)， epidermal growth factor receptor (EGFR)， AKT serine/threonine kinase (AKT1)， etc. Enrichment analysis suggested that Wubei Powder can regulate the cancer pathways， EGFR tyrosine kinase inhibitor resistance pathway， phosphatidylinositol-3 kinase/protein kinase B (PI3K-AKT) signaling pathway， and mitogen-activated protein kinase (MAPK) signaling pathway through biological processes such as positive regulation of MAP kinase activity， positive regulation of cell proliferation， negative regulation of cell apoptosis process， and response to exogenous stimuli. In molecular docking display， key targets (SRC EGFR， and AKT1) could freely bind with the main active ingredients (quercetin， geranium， and β -sitosterol)，and β-sitosterol had the strongest binding activity with SRC. Conclusion：The components of quercetin，geranium extract，and β-sitosterol in Wubei Powder can act on SRC EGFR，AKT1，and other targets，and regulate cancer pathways and the resistance pathway of EGFR tyrosine kinase inhibitors，thus playing a role in the treatment of RE and GU with different diseases，same treatment.