Abstract: Objective: To systematically evaluate the clinical effect of electroacupuncture in the treatment of post-stroke insomnia. Methods: The literature on randomized controlled trials (RCTs) using electroacupuncture as the main treatment for post-stroke insomnia was searched in databases such as the China National Knowledge Infrastructure (CNKI), Wangfang Data Knowledge Service Platform (Wanfang Data),Chinese Science and Technology Journal Database (VIP),China Biology Medicine (CBM),PubMed, Web of Science,Embase,and Cochrane Library,with a search deadline of October 31,2023. Data were extracted from the included literature and the included studies were evaluated by the Cochrane RoB 2.0 risk bias assessment tool; Meta-analysis was performed by Review Manager 5.4.1 software, and trial sequential analysis was performed by TSA 0.9.5.10 Beta software. Results: A total of eight studies were included, involving 516 patients. The Meta-analysis results showed that in terms of total effective rate, electroacupuncture combined with traditional acupuncture was superior to traditional acupuncture [RR= 1.14, 95%CI (1.03, 1.27), Z =2.41, P=0.02], and there was no significant difference between electroacupuncture and medication [RR=1.11,95%CI (0.92,1.33),Z =1.10,P=0.27],electroacupuncture combined with medication was superior to medication [RR=1.21,95%CI (1.02,1.43),Z =2.21,P=0.03]. In terms of reducing the total score of Pittsburgh Sleep Quality Index (PSQI),electroacupuncture combined with traditional acupuncture was superior to traditional acupuncture [MD=-1.61, 95%CI (-2.21, -1.01), Z =5.22, P<0.001],and there was no significant difference between electroacupuncture and medication [MD=-3.01, 95%CI (-6.61, 0.59),Z =1.64, P=0.10], electroacupuncture combined with medication was superior to medication [MD=-1.65, 95%CI (-2.02, -1.28),Z =8.85, P<0.001]. As for improving neurological deficits,electroacupuncture combined with traditional acupuncture was superior to traditional acupuncture [MD=-1.65,95%CI (-2.18,-1.12),Z =6.10,P<0.001]. In terms of improving depression, electroacupuncture combined with traditional acupuncture was superior to traditional acupuncture [MD= -1.42, 95%CI (-2.23, -0.61),Z =3.44, P<0.001]. After sensitivity analysis verification, the Metaanalysis results of the total effective rate in this study were stable. The trial sequential analysis results showed that in terms of total effective rate, the analysis results of electroacupuncture combined with traditional acupuncture superior to traditional acupuncture were robust, and the conclusion could exclude false positives. The analysis results of no significant difference between electroacupuncture and medication need to be included in more trials to verify. The analysis results of electroacupuncture combined with medication superior to medication need to be included in more trials to exclude false positives. Conclusion: In terms of the curative effect on post-stroke insomnia, the combination of electroacupuncture and traditional acupuncture is superior to traditional acupuncture, and electroacupuncture combined with medication is superior to medication. The conclusion requires more high-quality RCTs to support.