Abstract：Objective：To observe the clinical effect of acupuncture and umbilical moxibustion combined with Mesalazine on colitis of liver constraint and spleen deficiency type. Methods：A total of 120 cases of patients with colitis of liver constraint and spleen deficiency type were selected and divided into the control group 1， the control group 2， and the treatment group according to the random number table method， with 40 cases in each group. The control group 1 was treated with Mesalazine， the control group 2 was additionally treated with umbilical moxibustion based on the treatment of the control group 1， and the treatment group was additionally treated with acupuncture and umbilical moxibustion on the basis of the control group 1. The three groups were treated for four weeks and followed up for six months. Clinical effects，symptom scores，inflammatory factors [interleukin-6 (IL-6)，interleukin-10 (IL-10)，and tumor necrosis factor- α (TNF- α)]， the scores of the Hamilton Anxiety Scale (HAMA)， Hamilton Depression Rating Scale (HAMD) and Inflammatory Bowel Disease Queationaire (IBDQ)， recurrence rates， and incidence of adverse reactions were compared among the three groups. Results： After four-week treatment， the total effective rate was 95.00% in the treatment group， which was higher than that of 70.00% in the control group 1 and that of 77.50% in the control group 2 (P＜0.05). There was no significant difference in the comparison of the total effective rate between the control group 1 and the control group 2 (P＞0.05). After four-week treatment， the scores of stool containing pus and blood， abdominal pain， and diarrhea in the three groups were declined when compared with those before treatment (P＜0.05)；the scores of stool containing pus and blood，abdominal pain，and diarrhea in the treatment group were lower than those in the other two groups (P＜0.05)； the scores of stool containing pus and blood， abdominal pain，and diarrhea in the control group 2 were lower than those in the control group 1 (P＜0.05). After fourweek treatment，the levels of IL-6 and TNF-α in serum in the three groups were dropped when compared with those before treatment (P＜0.05)， and the serum IL-10 levels were increased when compared with those before treatment (P＜0.05)； the levels of IL-6 and TNF- α in serum in the treatment group were lower than those in the control group 1 and the control group 2 (P＜0.05)，and the serum IL-10 level in the treatment group were lower than those in the other two groups (P＜0.05)；the levels of IL-6 and TNF-α in serum in the control group 2 were lower than those in the control group 1 (P＜0.05)，and the serum IL-10 level in the control group 2 was lower than that in the control group 1 (P＜0.05). After four-week treatment， the scores of HAMA and HAMD in the three groups were decreased when compared with those before treatment (P＜0.05)， and the IBDQ scores were increased when compared with those before treatment (P＜0.05)； the scores of HAMA and HAMD in the treatment group were lower than those in the control group 1 and the control group 2 (P＜0.05)，and IBDQ scores in the treatment group were higher than those in the other two groups (P＜0.05)；the scores of HAMA and HAMD in the control group 2 were lower than those in the control group 1 (P＜0.05)，and the IBDQ scores in the control group 2 were higher than those in the control group 1 (P＜0.05). The recurrence rate and incidence of adverse reactions in the treatment group were 5.26% and 2.50%， respectively， which were lower than those in the control group 1 (53.57% and 25.00%，respectively) and the control group 2 (32.26% and 20.00%，respectively) (P＜0.05). There were no significant differences in the comparison of recurrence rate and incidence of adverse reactions between the control group 1 and the control group 2 (P＞0.05). Conclusion：Acupuncture and umbilical moxibustion combined with Mesalazine for colitis of liver constraint and spleen deficiency type can effectively improve symptoms， and negative emotions and quality of life， regulate the levels of inflammatory factors， and reduce recurrence rate，with good safety.