Abstract：Objective：To observe the clinical effect of acupuncture combined with repetitive transcranial magnetic stimulation in the recovery of upper limb dysfunction during the phase of flaccid paralysis after ischemic stroke. Methods： A total of 90 patients who underwent upper limb function recovery during the phase of flaccid paralysis after ischemic stroke were selected and divided into the study group and the control group by the random number table method，with 45 patients in each group．Both groups received basic treatment； the control group received repetitive transcranial magnetic stimulation in addition to the basic treatment，and the study group was additionally treated with acupuncture based on the treatment of the control group． Both groups were treated for four weeks． After four weeks of treatment， the clinical effects were observed． Before and after four weeks of treatment， the scores of Fugl-Meyer upper limb motor function，active range of motion of the joint，as well as serum levels of glial fibrillary acidic protein (GFAP)， myelin basic protein (MBP)， γ-aminobutyric acid (GABA)， and glutamate (Glu) were compared between two groups． The duration of flaccid paralysis and the rates of active wrist dorsiflexion were compared between the two groups. Results：After four weeks of treatment，the total effective rate in the study group was 95.56%， which was higher than that of 82.22% in the control group (P＜0.05)． both groups showed an increase in Fugl-Meyer upper limb motor function scores when compared with those before treatment (P＜0.05)， and the Fugl-Meyer upper limb motor function score in the study group was higher than that in the control group (P＜0.05). The range of motion for shoulder flexion， abduction， and extension， wrist dorsiflexion， palmar flexion， and elbow joint in the two groups was increased when compared with that before treatment (P＜0.05)；the range of motion for shoulder flexion，abduction，and extension，wrist dorsiflexion，palmar flexion，and elbow joint in the study group was biger than that in the control group (P＜0.05). The levels of serum GFAP， MBP， and Glu in the two groups were decreased when compared with those before treatment (P＜0.05)；the levels of serum GFAP，MBP，and Glu in the study group were lower than those in the control group (P＜0.05). The levels of serum GABA in the two groups were increased when compared with those before treatment (P＜0.05)；the levels of serum GABA in the study group were higher than those in the control group (P＜0.05). The duration of flaccid paralysis in the study group was shorter than that in the control group (P＜0.05). The active wrist dorsiflexion rate in the study group was 91.11%，which was higher than that of 68.89% in the control group (P＜0.05). Conclusion： Acupuncture combined with repetitive transcranial magnetic stimulation can ischemic help stroke patients be free from flaccid paralysis， promote active limb movement and the reconstruction of upper limb function，and reduce the impairment of the central nervous system ．