Abstract：Objective：To study the correlation between inflammation markers of white blood cell (WBC) count， hypersensitive C-reactive protein (hs-CRP) and erythrocyte sedimentation rate (ESR) of children with fever of unknown origin (FUO) and eight-principle syndrome types. Methods： Retrospectively analyzed the clinical data of 128 children who were treated in the Pediatrics Inpatient Unit of the First Affiliated Hospital of Guangzhou University of Chinese Medicine from January 2018 to December 2023 and compatible with the diagnosis of FUO. The data on WBC，hs-CRP，ESR and traditional Chinese medicine syndromes were obtained. Based on the data on four diagnostic methods，they were divided into four types of exterior syndrome， interior syndrome， half-exterior half-interior syndrome， and dual disease of the exterior and interior according to the two principles of the exterior and interior of eight principle syndrome differentiation； there were four types of cold syndrome， heat syndrome， cold and heat complex syndrome， and no obvious cold-heat syndrome according to the two principles of cold and heat； there were three types of deficiency syndrome， excess syndrome and deficiency-excess complex syndrome according to the two principles of deficiency and excess (yin and yang are the general principles，leading the other six principles， so they were not included in this discussion). The differences of the above three inflammation markers of various syndrome types were compared. Results：There was significant difference being found in the comparison of hs-CRP level among the four syndrome types of the two principles of the exterior and interior (P＜0.05)；the hs-CRP level of exterior syndrome was lower than that of dual disease of the exterior and interior， the difference being significant (P＜0.05). There were significant differences being found in the respective comparisons of levels of WBC and ESR among the four syndrome types of the two principles of cold and heat (P＜0.05)； the WBC level of cold syndrome was lower than that of heat syndrome，the difference being significant (P＜0.05). There were significant differences being found in the respective comparisons of levels of WBC and ESR among the three syndrome types of the two principles of deficiency and excess (P＜0.05)； the ESR level of excess syndrome was lower than that of deficiencyexcess complex syndrome， the difference being significant (P＜0.05). Conclusion： The inflammation markers of WBC， hs-CRP and ESR have certain reference significance for the eight principle syndrome differentiation of FUO in children. Among them，the hs-CRP is closely related to the exterior and interior of the disease location，and the WBC and ESR are closely related to the cold and heat，and the deficiency and excess of the disease nature.