Abstract： Objective： To study the effect of the therapy of injection with 50% glucose at Neiguan （PC6） point combined with low end tidal carbon dioxide （LEtCO2） on postoperative nausea and vomiting （PONV） after gynecological hysteroscopy-laparoscopy. Methods：A total of 90 female patients undergoing hysteroscopy and laparoscopy in Jinhua Central Hospital Affiliated to Zhejiang University School of Medicine from January 2023 to April 2023 were included and divided into the Ondansetron group， the acupoint injection group and the combination group according to the random number table method， with 30 cases in each group. The Ondansetron group was treated with a single intravenous injection of Ondansetron Hydrochloride， and controlled ventilation mode was used at the beginning of surgery. The acupoint injection group was treated with 50% glucose injection at the bilateral Neiguan （PC6） points，and controlled ventilation mode was used. In the combination group， 50% glucose injection at bilateral Neiguan （PC6） points was combined with LEtCO2 for treatment. The incidence of PONV，severity of nausea and vomiting，use of remedial drugs， regional cerebral oxygen saturation （rSO2）， Visual Analogue Scale （VAS） scores， total hospitalization time， first feeding time，and first exhaustion or defecation time were compared among the three groups. Results：The incidence of PONV during the first 2 hours in the combination group was lower than that in the Ondansetron group （P＜0.05）. The incidence of PONV during the 2 to 4 hours，and the 5 to 24 hours after operation and the proportion of patients using additional antiemetic medicines respectively in the combination group were lower than those in the Ondansetron group and the point injection group（ P＜0.05）. The percentage of patients with grade Ⅰand Ⅲ nausea and vomiting during the first 24 hours after operation in the combination group was higher than that in the Ondansetron group and the point injection group （P＜0.05）. The length of hospital stay in the combination group was shorter than that in the Ondansetron group and the point injection group， respectively （P＜0.05）. Time of first food intake， and time of first exhaust or defecation in the combination group were earlier than those in the Ondansetron group and the point injection group （P＜ 0.05）. When compared with that before anesthesia implementation （T0）， rSO2 levels were increased 5 minutes after intubation during anesthesia （T1），before the return to the Trendellenberg position （T2），and 5 minutes after the return to the lithotomy position （T3） in all three groups （P＜0.05）; the rSO2 levels at T2 and T3 in the combination group were lower than those in the Ondansetron group and the point injection group during the same period （P ＜ 0.05）. At 2 hours （T4），4 hours （T5），12 hours （T6），and 24 hours （T7） after operation，there was no significant difference in VAS scores among the three groups at the same time points （P＞0.05）. Conclusion：The therapy of injection with 50% glucose at Neiguan（ PC6） point combined with LEtCO2 can reduce the incidence and severity of PONV in the early stage of hysteroscopy and laparoscopy and the adverse effects of PONV on perioperative rehabilitation.