Abstract： Objective： To observe effect of baicalein on the expression of PI3K/Akt signaling pathway related factors in HT22 cells induced by Aβ25-35. Methods：The Aβ25-35 at 40 μmol·L-1 was used to intervened in HT22 cells for 24 hours to establish a dementia cell model. Four different concentrations（5，10，20，40 μmol·L-1） of baicalein were used to intervene in dementia cell models for 24 hours. The cell viability was detected by CCK-8 method， and the baicalein concentration with the highest cell viability was selected for subsequent trials. The trial models were divided into the control group，the Aβ25-35 group，and the baicalein group. Flow cytometry and Hoechest 33342 staining were used to detect cell apoptosis；JC-10 method was used to detect mitochondrial membrane potential；DCFH-DA method was used to detect levels of reactive oxygen species； Immunofluorescence was used to detect microtubule associated protein light chain 3 （LC3） protein expression；Western blot was used to detect the expression of selective autophagy adaptor protein（p62）， autophagy key molecule yeast Atg6 homolog 1（Beclin-1）， phosphatidylinositol 3- kinase（PI3K）， phosphorylated phosphatidylinositol 3-kinase （p-PI3K）， serine/threonine protein kinase （Akt）， phosphorylated serine/threonine protein kinase （p-Akt）， and mammalian target of rapamycin （mTOR）， and compared with the PI3K specific inhibitor LY294002. Results：Finally，a concentration of 10 μmol·L-1 of baicalein was chosen for the subsequent trial. Compared with those in the control group， the apoptosis rate， ROS level， p62 protein expression，and mTOR protein expression were increased in the Aβ25-35 group （P＜0.05），while MMP level， LC3 expression，Beclin-1 protein expression，p-PI3K/PI3K ratio，and p-Akt/Akt ratio were decreased （P＜0.05）； Compared with those in the Aβ25-35 group， the cell apoptosis rate， ROS level， p62 protein expression， and mTOR protein expression in the baicalein group were decreased （P＜0.05），while MMP levels，LC3 expression，Beclin-1 protein expression， p-PI3K/PI3K ratio， and p-Akt/Akt ratio were raised （P＜0.05） . Compared with those in the baicalein group，the addition of PI3K inhibitor LY294002 resulted in a decrease in the ratio of p-PI3K/PI3K and p-Akt/ Akt，and an increase in mTOR protein expression （P＜0.05） . Conclusion：Baicalein can significantly improve the mitochondrial function of HT22 cells induced by Aβ25-35， and reduce cell apoptosis and oxidative stress. Its antidementia effect is related to the activation of the PI3K/AKT signaling pathway and the increase of cellular autophagy levels.