Abstract： Objective： To observe the clinical effect of the combination use of Zishen Shugan Prescription and sequential therapy of estrogen and progesterone on premature ovarian failure of kidney deficiency and liver constraint type. Methods： A total of 86 patients with premature ovarian failure of kidney deficiency and liver constraint type admitted to General Hospital of Pingmei Shenma Group from January 2021 to December 202 were divided into the control group and the observation group by the random number table method，with 4 cases in each group. The control group was treated with sequential therapy of estrogen and progesterone， and the observation group was treated with Zishen Shugan Decoction based on the treatment of the control group. Both groups were treated for six weeks. The traditional Chinese medicine（TCM） syndrome scores， ovarian volume， antral follicle count and the levels of sex hormones before and after treatment， and the clinical effects and the incidence of adverse reactions were compared between the two groups. Results：The total effective rate was 88.7% （8/4） in the observation group，higher than that of 69.77% （0/4） in the control group （P＜0.05）. After treatment， the TCM syndrome scores and the levels of serum follicle stimulating hormone （FSH） and luteinizing hormone （LH） in the two groups were dwindled when compared with those before treatment （P＜0.05），and the E2 levels，ovarian volume and the number of antral follicles were elevated （P＜0.05）； after treatment， the TCM syndrome scores and the levels of serum FSH and LH in the observation group were lower than those in the control group （P＜0.05）， and the E2 level， ovarian volume and the number of antral follicles were respectively higher than those in the control group （P＜0.05）. There was no significant difference being found in the comparison of the incidence of adverse reactions between the two groups （P＞0.05）. Conclusion：The combination use of Zishen Shugan Prescription and sequential therapy of estrogen and progesterone has a definite curative effect on premature ovarian failure of kidney deficiency and liver constraint type， which can adjust the levels of sex hormones and ovarian volume of patients with great safety.