Abstract： Objective： To observe the effects of acupuncture intervention on ovarian function and autophagyrelated proteins in ovarian tissue of rats with premature ovarian failure. Methods： Female Wistar rats were randomly divided into a blank group，a model group，a medication group，a core acupoints group，and a non-meridian and non acupoint group. All rats except for the blank group were injected intraperitoneally with cyclophosphamide to establish a rat model of premature ovarian failure. Rats in the medication group were given diethylstilbestrol by gavage； the core acupoints group was treated with acupuncture at the Guanyuan （CV4） point and bilateral Sanyinjiao （SP6） points；the non-meridian and non-acupoint group was given acupuncture at locations that were neither on the meridians nor acupoints and 5 mm away from the Guanyuan （CV4） point and bilateral Sanyinjiao （SP6） points；the blank group and the model group were given the same treatment without any intervention factors. After 14 days， samples were taken， rats were weighed， and ovarian and uterine organ indicators were calculated. HE staining was used to observe the morphology of the ovaries and uterus， and the follicles and corpus luteum were counted at all levels. Enzyme-linked immunosorbent assay （ELISA） was used to detect the levels of serum estradiol （E2） ， follicle-stimulating hormone（ FSH），luteinizing hormone（ LH），and anti-Müllerian hormone（ AMH）. Western blot was used to detect the expression levels of PTEN，AKT，p-AKT，LC，and Beclin1 proteins in ovarian tissue. The RT-qPCR was used to detect the relative mRNA expression levels of PTEN，AKT，LC，and Beclin1 in ovarian tissue. Results：Compared with the blank group，the body mass，and ovarian and uterine organ indicators in the model group were decreased （P＜ 0.05）； the number of growing follicles and corpus luteum was decreased， and the number of blocked follicles was increased （P＜0.05）； the serum AMH and E2 levels were declined， and FSH and LH levels were increased （P＜ 0.05）；the protein and mRNA expression levels of PTEN，LC，and Beclin1 in ovarian tissue were upregulated，and the p-AKT/AKT level was downregulated （P＜0.05）. Compared with the model group，the core acupoints group showed an increase in body mass and ovarian and uterine organ indicators （P＜0.05），an increase in the number of primordial follicles， and a decrease in the number of blocked follicles （P＜0.05）； the serum AMH and E2 levels in the core acupoints group were enhanced，and FSH and LH levels were declined （P＜0.05）；the protein and mRNA expression levels of PTEN，LC，and Beclin1 in ovarian tissue in the core acupoints group were downregulated，and the p-AKT/ AKT level was upregulated （P＜0.05）. Compared with the non-meridian and non-acupoint group，the core acupoints group showed an increase in body mass and ovarian and uterine organ indicators （P＜0.05），an increase in the number of primordial follicles and a decrease in the number of blocked follicles （P＜0.05），an increase in serum AMH and E2 levels，a decrease in LH levels （P＜0.05），and a downregulation of Beclin1 protein expression in ovarian tissue （P＜ 0.05）. Conclusion：Acupuncture at the core acupoints of the Guanyuan （CV4） point and bilateral Sanyinjiao （SP6） points can regulate serum hormone levels in rats， restore ovarian function， and reduce the expression of LC and Beclin1 proteins and mRNA in ovarian tissue of rats with premature ovarian failure by affecting the PTEN/AKT signaling pathway. It also inhibits excessive autophagy damage of ovarian granulosa cells and reduces follicular atresia.