Abstract： Objective： To explore the action mechanism of Fanlian Huazhuo Granules（FHK） for ulcerative colitis （UC） by network pharmacology combined with Gene Expression Omnibus （GEO） gene chip analysis. Methods： The active components and corresponding target genes of FHK were obtained from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform （TCMSP） and the HERB database； Cytoscape software was used to construct an "active component-drug target" regulatory network； differentially expressed genes for UC were obtained from the GEO database；intersection targets were identified by combining differentially expressed genes of the disease with the active components of the drug，and a protein-protein interaction （PPI） network was constructed using the STRING database； Gene Ontology （GO） functional and Kyoto Encyclopedia of Genes and Genomes （KEGG） pathway enrichment analyses were performed on the intersection targets； based on the screened core components and core targets，molecular docking verification was conducted by Autodock Vina software to preliminarily verify the binding affinity between core components and targets. Results： A total of 83 active components of FHK were screened， corresponding to 1495 targets；GEO data revealed 973 differentially expressed genes for UC；there were 41 intersection targets being identified as potential targets for FHK in the treatment of UC. GO and KEGG results indicated that FHK primarily affected biological processes such as the response to lipopolysaccharides， response to bacterial molecules， activation of cytokines， and mediation of signaling pathways. These processes mainly occurred in membrane rafts and membrane microdomains and are closely related to tumor necrosis factor，lipid and atherosclerosis，and interleukin-17 signaling pathways. Molecular docking simulation results showed a high binding affinity between the active components of FHK and the core targets. Conclusion： The treatment of UC with FHK is achieved through multi-target， multipathway， and multi-process interactions. The primary action mechanism is likely focused on anti-inflammation and immune regulation.