Abstract：Objective：To observe the clinical effect of the combination use of warming-needle moxibustion and infrared irradiation for frozen shoulder with wind-cold-damp obstruction syndrome and its effect on bradykinin （BK）， 5-hydroxytryptamine （5-HT） and prostaglandin E2 （PGE2）. Methods：A total of 94 patients with frozen shoulder of wind-cold-damp obstruction syndrome treated in Wenzhou TCM Hospital of Zhejiang Chinese Medical University from May 2023 to May 2024 were selected and randomly divided into the control group and the trial group by computer software，with 47 cases in each group. The control group was treated with infrared irradiation，and the trial group was treated with warming-needle moxibustion combined with infrared irradiation. Both groups were treated for four weeks. The clinical effects， scores of Constant-Murley Score （CMS）， Shoulder Pain and Disability Index （SPADI）， and levels of serum BK，5-HT and PGE2 were compared between the two groups；the adverse reactions during treatment were recorded. Results：After four weeks of treatment，the total effective rate was 91.49% （43/47） in the trial group， higher than that of 74.47%（ 35/47） in the control group，the difference being significant（ P＜0.05）. After four weeks of treatment，the CMS scores in the two groups were elevated when compared with those before treatment，and the SPADI scores were reduced when compared with those before treatment， differences being significant （P＜0.05）. The CMS score in the trial group was higher than that in the control group，and the SPADI score was lower than that in the control group， differences being significant （P＜0.05）. After four weeks of treatment， the levels of serum BK， 5-HT and PGE2 in the two groups were down-regulated when compared with those before treatment， differences being significant （P＜0.05）；the above three levels in the trial group were lower than those in the control group，differences being significant （P＜0.05）. During the treatment，the incidence of adverse reactions was 10.64% （5/47） in the trial group，and 6.38% （3/47） in the control group，there being no significance in the difference （P＞0.05）. Conclusion： Compared with the single use of infrared irradiation，the combination use of warming-needle moxibustion and infrared irradiation can improve the curative effect on frozen shoulder with wind-cold-damp obstruction syndrome，relieve the pain，improve the shoulder joint function，reduce the level of pain medium with great safety.