Abstract： Objective： To analyze the potential mechanism of action of Stemonae Radix in treating whooping cough through network pharmacology and molecular docking techniques. Methods： The effective components of Stemonae Radix were screened via the TCMSP database and supplemented with the HERB database. The targets of Stemonae Radix were obtained via UniProt， PubMed， and SwissTargetPrediction databases， while whooping coughrelated targets were obtained via GeneCards，OMIM，DisGeNET，TTD，and Drugbank databases. The intersection of these targets was taken as the "medicine-disease" targets，and a protein-protein interaction network was constructed via the STRING database. Gene Ontology （GO） and Kyoto Encyclopedia of Genes and Genomes （KEGG） pathway analyses were performed using David and KEGG databases， and corresponding networks were constructed via Cytoscape software. Molecular docking of effective components with key targets was performed via AutoDockTools software to validate the results of the network pharmacological study. Results： A total of 53 effective components of Stemonae Radix were obtained，involving 752 targets，and 1 507 whooping cough-related targets，with 285 intersected targets. Frequency analysis of the protein-protein interaction network revealed that the mechanism of Stemonae Radix in treating whooping cough is related to targets such as SRC，AKT1，STAT3，CASP3，and EGFR. GO and KEGG enrichment analyses showed that multiple signaling pathways such as neuroactive ligand-receptor interaction，pathways in cancer， calcium signaling pathway，lipids and atherosclerosis signaling pathway，cAMP signaling pathway，and proteoglycans in cancer may play important roles in the treatment of whooping cough with Stemonae Radix. Molecular docking results indicated good binding interactions between these active components and targets. Conclusion： Stemonae Radix may exert anti-inflammatory effects by acting on targets such as SRC，AKT1，STAT3，CASP3，and EGFR through active components such as β -sitosterol， sesamin， and formononetin， and regulating neuroactive ligand-receptor interactions，cancer signaling pathways，calcium signaling pathways，and other pathways.