Abstract: Objective: To analyze the studies on acupuncture and moxibustion for ankylosing spondylitis (AS) and to explore the research hotspots and trends in this field. Methods: The clinical studies on acupuncture and moxibustion for AS collected by China National Knowledge Infrastructure (CNKI),Wanfang Data Knowledge Service Platform (Wanfang Data) and China Science and Technology Journal Database (VIP) from January 1st, 1994 to January 31st, 2024 was searched. Using NoteExpress v4.0 software and manual screening, the studies were deduplicated. VOSviewer 1.6.20 software was used for the co-occurrence analysis on the authors and keywords. CiteSpace 6.3. R1 software was used for the analysis on cluster, timeline and bursts of keywords, and the visual knowledge map was drawn. Results:A total of 636 studies were included. The number of studies published in this field increased year by year,involving 1 319 authors,forming a research team represented by LIU Wei,ZHOU Youlong, LI Li,SONG Min and LI Li. The keywords of the studies formed nine meaningful clusters,indicating that the research hotspots in this field mainly focused on acupuncture, warming-needle moxibustion, curative effect, Chinese medicinals,inflammatory factors,etc. The main intervention approaches of acupuncture and moxibustion are warmingneedle moxibustion, moxibustion at governor vessel, electroacupuncture, fire needling, acupoint injection, medicine-separated moxibustion, blood-letting puncture and cupping, etc. The main acupuncture and moxibustion therapies are arrayed needling, long-snake moxibustion, central-square needling, point pricking with fire needle, and long-snake moxibustion at governor vessel, mainly focusing on clinical effect, functional index, joint activity, pain,time of morning stiffness,quality of life,inflammation index,etc. Conclusion:In recent years,the research on acupuncture and moxibustion for AS has been reduced,and the core research team has not yet been formed. There are few basic experimental studies, and academic exchanges and cooperation need to be strengthened. The research hotspots mainly involve different acupuncture and moxibustion therapies, clinical effect and evaluation indicators, mechanism,combined modality therapy of Chinese medicinals,etc.